Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (76)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tieman, D. M.
Right arrow Articles by Handa, A. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tieman, D. M.
Right arrow Articles by Handa, A. K.
Agricola
Right arrow Articles by Tieman, D. M.
Right arrow Articles by Handa, A. K.

PLANT PHYSIOLOGY , Vol 106, Issue 2 429-436, Copyright © 1994 by American Society of Plant Biologists

MOLECULAR BIOLOGY AND GENE REGULATION

Reduction in Pectin Methylesterase Activity Modifies Tissue Integrity and Cation Levels in Ripening Tomato (Lycopersicon esculentum Mill.) Fruits

D. M. Tieman and A. K. Handa
Department of Horticulture, Purdue University, 1165 Horticulture Building, West Lafayette, Indiana 47907-1165

Pectin methylesterase (PME, EC 3.1.1.11) is an ubiquitous enzyme in the plant kingdom; however, its role in plant growth and development is not yet understood. Using transgenic tomato (Lycopersicon esculentum Mill.) fruits that show more than 10-fold reduction in PME activity because of expression of an antisense PME gene, we have investigated the role of PME in tomato fruit ripening. Our results show that reduced PME activity causes an almost complete loss of tissue integrity during fruit senescence but shows little effect on fruit firmness during ripening. Low PME activity in the transgenic fruit pericarp modified both accumulation and partitioning of cations between soluble and bound forms and selectively impaired accumulation of Mg2+ over other major cations. Decreased PME activity was associated with a 30 to 70% decrease in bound Ca2+ and Mg2+ in transgenic pericarp. Levels of soluble Ca2+ increase 10 to 60%, whereas levels of soluble Mg2+ and Na+ are reduced by 20 to 60% in transgenic pericarp. Changes in cation levels associated with lowered PME activity do not affect the rate of respiration or membrane integrity of fruit during ripening. Overall, these results suggest that PME plays a role in determining tissue integrity during fruit senescence, perhaps by regulating cation binding to the cell wall.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
T. D. Phan, W. Bo, G. West, G. W. Lycett, and G. A. Tucker
Silencing of the Major Salt-Dependent Isoform of Pectinesterase in Tomato Alters Fruit Softening
Plant Physiology, August 1, 2007; 144(4): 1960 - 1967.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
V. Lionetti, A. Raiola, L. Camardella, A. Giovane, N. Obel, M. Pauly, F. Favaron, F. Cervone, and D. Bellincampi
Overexpression of Pectin Methylesterase Inhibitors in Arabidopsis Restricts Fungal Infection by Botrytis cinerea
Plant Physiology, April 1, 2007; 143(4): 1871 - 1880.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. Bosch and P. K. Hepler
Pectin Methylesterases and Pectin Dynamics in Pollen Tubes
PLANT CELL, December 1, 2005; 17(12): 3219 - 3226.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
T.-W. Wang, C.-G. Zhang, W. Wu, L. M. Nowack, E. Madey, and J. E. Thompson
Antisense Suppression of Deoxyhypusine Synthase in Tomato Delays Fruit Softening and Alters Growth and Development
Plant Physiology, July 1, 2005; 138(3): 1372 - 1382.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Bosch, A. Y. Cheung, and P. K. Hepler
Pectin Methylesterase, a Regulator of Pollen Tube Growth
Plant Physiology, July 1, 2005; 138(3): 1334 - 1346.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
A. Di Matteo, A. Giovane, A. Raiola, L. Camardella, D. Bonivento, G. De Lorenzo, F. Cervone, D. Bellincampi, and D. Tsernoglou
Structural Basis for the Interaction between Pectin Methylesterase and a Specific Inhibitor Protein
PLANT CELL, March 1, 2005; 17(3): 849 - 858.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. M. Eriksson, A. Bovy, K. Manning, L. Harrison, J. Andrews, J. De Silva, G. A. Tucker, and G. B. Seymour
Effect of the Colorless non-ripening Mutation on Cell Wall Biochemistry and Gene Expression during Tomato Fruit Development and Ripening
Plant Physiology, December 1, 2004; 136(4): 4184 - 4197.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Guan and E. A. Nothnagel
Binding of Arabinogalactan Proteins by Yariv Phenylglycoside Triggers Wound-Like Responses in Arabidopsis Cell Cultures
Plant Physiology, July 1, 2004; 135(3): 1346 - 1366.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Castillejo, J. I. de la Fuente, P. Iannetta, M. A. Botella, and V. Valpuesta
Pectin esterase gene family in strawberry fruit: study of FaPE1, a ripening-specific isoform
J. Exp. Bot., April 1, 2004; 55(398): 909 - 918.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Ramakrishna, Z. Deng, C.-K. Ding, A. K. Handa, and R. H. Ozminkowski Jr.
A Novel Small Heat Shock Protein Gene, vis1, Contributes to Pectin Depolymerization and Juice Viscosity in Tomato Fruit
Plant Physiology, February 1, 2003; 131(2): 725 - 735.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Alexander and D. Grierson
Ethylene biosynthesis and action in tomato: a model for climacteric fruit ripening
J. Exp. Bot., October 1, 2002; 53(377): 2039 - 2055.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. L. Smith, J. A. Abbott, and K. C. Gross
Down-Regulation of Tomato beta -Galactosidase 4 Results in Decreased Fruit Softening
Plant Physiology, August 1, 2002; 129(4): 1755 - 1762.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Orfila, G. B. Seymour, W. G.T. Willats, I. M. Huxham, M. C. Jarvis, C. J. Dover, A. J. Thompson, and J. P. Knox
Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1{right-arrow}5)-{alpha}-L-Arabinan in the Pericarp of Cnr, a Ripening Mutant of Tomato
Plant Physiology, May 1, 2001; 126(1): 210 - 221.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
C. Ren and A. R. Kermode
An Increase in Pectin Methyl Esterase Activity Accompanies Dormancy Breakage and Germination of Yellow Cedar Seeds
Plant Physiology, September 1, 2000; 124(1): 231 - 242.
[Abstract] [Full Text]

Home page
 Plant CellHome page
D. A. Brummell, M. H. Harpster, P. M. Civello, J. M. Palys, A. B. Bennett, and P. Dunsmuir
Modification of Expansin Protein Abundance in Tomato Fruit Alters Softening and Cell Wall Polymer Metabolism during Ripening
PLANT CELL, November 1, 1999; 11(11): 2203 - 2216.
[Abstract] [Full Text]

Home page
 Plant CellHome page
F. Wen, Y. Zhu, and M. C. Hawes
Effect of Pectin Methylesterase Gene Expression on Pea Root Development
PLANT CELL, June 1, 1999; 11(6): 1129 - 1140.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
C. Frenkel, J. S. Peters, D. M. Tieman, M. E. Tiznado, and A. K. Handa
Pectin Methylesterase Regulates Methanol and Ethanol Accumulation in Ripening Tomato (Lycopersicon esculentum) Fruit
J. Biol. Chem., February 20, 1998; 273(8): 4293 - 4295.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1994 by the American Society of Plant Biologists