Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ross, G. S.
Right arrow Articles by Redgwell, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ross, G. S.
Right arrow Articles by Redgwell, R. J.
Agricola
Right arrow Articles by Ross, G. S.
Right arrow Articles by Redgwell, R. J.

PLANT PHYSIOLOGY , Vol 106, Issue 2 521-528, Copyright © 1994 by American Society of Plant Biologists

METABOLISM AND ENZYMOLOGY

Apple [beta]-Galactosidase (Activity against Cell Wall Polysaccharides and Characterization of a Related cDNA Clone)

G. S. Ross, T. Wegrzyn, E. A. MacRae and R. J. Redgwell
The Horticulture and Food Research Institute of New Zealand Ltd., Mt. Albert Research Centre, Private Bag 92 169, Auckland, New Zealand

A [beta]-galactosidase was purified from cortical tissue of ripe apples (Malus domestica Borkh. cv Granny Smith) using a procedure involving affinity chromatography on lactosyl-Sepharose. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that two polypeptides of 44 and 32 kD were present in the fraction that showed activity against the synthetic substrate p-nitrophenol-[beta]-D-galactopyranoside. The enzyme preparation was incubated with polysaccharide extracts from apple cell walls containing [beta]-(1->4)-linked galactans, and products of digestion were analyzed by gas chromatography. Small amounts of monomeric galactose were released during incubation, showing that the enzyme was active against native substrates. Amino acid sequence information was obtained from the purified protein, and this showed high homology with the anticipated polypeptide coded by the ethylene-regulated SR12 gene in carnation (K.G. Raghothama, K.A. Lawton, P.B. Goldborough, W.R. Woodson [1991] Plant Mol Biol 17: 61-71) and a harvest-related pTIP31 cDNA from asparagus (G. King, personal communication). Using the asparagus cDNA clone as a probe, an apple homolog (pABG1) was isolated. This clone contains a 2637-bp insert, including an open reading frame that codes for a polypeptide of 731 amino acids. Cleavage of an N-terminal signal sequence would leave a predicted polypeptide of 78.5 kD. Genomic DNA analysis and the isolation of other homologous apple clones suggest that pABG1 represents one member of an apple [beta]-galactosidase gene family. Northern analysis during fruit development and ripening showed accumulation of pABG1-homologous RNA during fruit ripening. Enzyme activity as measured in crude extracts increased during fruit development to a level that was maintained during ripening.


This article has been cited by other articles:


Home page
 jashsHome page
H. S. Mann, J. J. Alton, S. Kim, and C. B.S. Tong
Differential Expression of Cell-wall Modifying Genes and Novel cDNAs in Apple Fruit During Storage
J. Amer. Soc. Hort. Sci., January 1, 2008; 133(1): 152 - 157.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E.-J. Lee, Y. Matsumura, K. Soga, T. Hoson, and N. Koizumi
Glycosyl Hydrolases of Cell Wall are Induced by Sugar Starvation in Arabidopsis
Plant Cell Physiol., March 1, 2007; 48(3): 405 - 413.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Kotake, S. Dina, T. Konishi, S. Kaneko, K. Igarashi, M. Samejima, Y. Watanabe, K. Kimura, and Y. Tsumuraya
Molecular Cloning of a {beta}-Galactosidase from Radish That Specifically Hydrolyzes {beta}-(1->3)- and {beta}-(1->6)-Galactosyl Residues of Arabinogalactan Protein
Plant Physiology, July 1, 2005; 138(3): 1563 - 1576.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Z. Wu and J. K. Burns
A {beta}-galactosidase gene is expressed during mature fruit abscission of 'Valencia' orange (Citrus sinensis)
J. Exp. Bot., July 1, 2004; 55(402): 1483 - 1490.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. Tanaka, T. Fukui, H. Atomi, and T. Imanaka
Characterization of an Exo-{beta}-D-Glucosaminidase Involved in a Novel Chitinolytic Pathway from the Hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1
J. Bacteriol., September 1, 2003; 185(17): 5175 - 5181.
[Abstract] [Full Text] [PDF]

Home page
 Food Science and Technology InternationalHome page
M. Serrano, A. Amoros, M.T. Pretel, M.C. Martinez-Madrid, R. Madrid, and F. Romojaro
Effect of Calcium Deficiency on Melon (Cucumis melo L.) Texture and Glassiness Incidence During Ripening
Food Science and Technology International, June 1, 2002; 8(3): 147 - 154.
[Abstract] [PDF]

Home page
 J Exp BotHome page
L. Trainotti, R. Spinello, A. Piovan, S. Spolaore, and G. Casadoro
{beta}-Galactosidases with a lectin-like domain are expressed in strawberry
J. Exp. Bot., August 1, 2001; 52(361): 1635 - 1645.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
A. Tateishi, H. Inoue, H. Shiba, and S. Yamaki
Molecular Cloning of {beta}-Galactosidase from Japanese Pear (Pyrus pyrifolia) and its Gene Expression with Fruit Ripening
Plant Cell Physiol., May 1, 2001; 42(5): 492 - 498.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
H. J. Rogers, S. L. Maund, and L. H. Johnson
A {beta}-galactosidase-like gene is expressed during tobacco pollen development
J. Exp. Bot., January 1, 2001; 52(354): 67 - 75.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. K.C. Rose, K. A. Hadfield, J. M. Labavitch, and A. B. Bennett
Temporal Sequence of Cell Wall Disassembly in Rapidly Ripening Melon Fruit
Plant Physiology, June 1, 1998; 117(2): 345 - 361.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
D. L. Smith, D. A. Starrett, and K. C. Gross
A Gene Coding for Tomato Fruit beta -Galactosidase II Is Expressed during Fruit Ripening . Cloning, Characterization, and Expression Pattern
Plant Physiology, June 1, 1998; 117(2): 417 - 423.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1994 by the American Society of Plant Biologists