Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (37)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Funk, C.
Right arrow Articles by Croteau, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Funk, C.
Right arrow Articles by Croteau, R.
Agricola
Right arrow Articles by Funk, C.
Right arrow Articles by Croteau, R.

PLANT PHYSIOLOGY , Vol 106, Issue 3 999-1005, Copyright © 1994 by American Society of Plant Biologists

ENVIRONMENTAL AND STRESS PHYSIOLOGY

Regulation of Oleoresinosis in Grand Fir (Abies grandis) (Coordinate Induction of Monoterpene and Diterpene Cyclases and Two Cytochrome P450-Dependent Diterpenoid Hydroxylases by Stem Wounding)

C. Funk, E. Lewinsohn, B. S. Vogel, C. L. Steele and R. Croteau
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340

Oleoresin (pitch) is a defensive secretion composed of monoterpene olefins (turpentine) and diterpene resin acids (rosin) that is produced in grand fir (Abies grandis Lindl.) stems in response to wounding. Monoterpene and diterpene biosynthesis are coordinately induced in wounded stems as determined by monitoring the activity of monoterpene and diterpene cyclases, as well as two cytochrome P450-dependent diterpenoid hydroxylases involved in the formation of ([mdash])-abietic acid, the principal resin acid of this species. The activity of these enzymes reaches maximum levels that are 5- to 100-fold higher than those of nowwounded control stems 10 d after wounding and this is followed by a synchronous decline. The increase in biosynthetic activity is consequently followed by the accumulation of a viscous mass of resin acids, with the loss of the volatile monoterpenes, at the site of injury. The observed coordinate induction of monoterpene olefin and abietic acid bio-synthesis and the results of oleoresin analysis are consistent with the role of the volatile monoterpenes as a solvent for the mobilization and deposition of resin acids at the wound site to seal the injury with a rosin barrier after the evaporation of the turpentine. The last step of resin acid biosynthesis is catalyzed by an operationally soluble aldehyde dehydrogenase that is not inducible by wounding but seemingly is expressed constitutively at a high level. In vivo [14C]acetate feeding and resin analysis indicate that this enzyme is not efficiently coupled to the earlier steps of the pathway.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
A. Byun-McKay, K.-A. Godard, M. Toudefallah, D. M. Martin, R. Alfaro, J. King, J. Bohlmann, and A. L. Plant
Wound-Induced Terpene Synthase Gene Expression in Sitka Spruce That Exhibit Resistance or Susceptibility to Attack by the White Pine Weevil
Plant Physiology, March 1, 2006; 140(3): 1009 - 1021.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D.-K. Ro, G.-I. Arimura, S. Y. W. Lau, E. Piers, and J. Bohlmann
Loblolly pine abietadienol/abietadienal oxidase PtAO (CYP720B1) is a multifunctional, multisubstrate cytochrome P450 monooxygenase
PNAS, May 31, 2005; 102(22): 8060 - 8065.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. M. Martin, J. Gershenzon, and J. Bohlmann
Induction of Volatile Terpene Biosynthesis and Diurnal Emission by Methyl Jasmonate in Foliage of Norway Spruce
Plant Physiology, July 1, 2003; 132(3): 1586 - 1599.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. J. Bouwmeester, F. W.A. Verstappen, M. A. Posthumus, and M. Dicke
Spider Mite-Induced (3S)-(E)-Nerolidol Synthase Activity in Cucumber and Lima Bean. The First Dedicated Step in Acyclic C11-Homoterpene Biosynthesis
Plant Physiology, September 1, 1999; 121(1): 173 - 180.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
H. J. Bouwmeester, J. Gershenzon, M. C.J.M. Konings, and R. Croteau
Biosynthesis of the Monoterpenes Limonene and Carvone in the Fruit of Caraway . I. Demonstration of Enzyme Activities and Their Changes with Development
Plant Physiology, July 1, 1998; 117(3): 901 - 912.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Bohlmann, J. Crock, R. Jetter, and R. Croteau
Terpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible (E)-alpha -bisabolene synthase from grand fir (Abies grandis)
PNAS, June 9, 1998; 95(12): 6756 - 6761.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. L. Steele, S. Katoh, J. Bohlmann, and R. Croteau
Regulation of Oleoresinosis in Grand Fir (Abies grandis) . Differential Transcriptional Control of Monoterpene, Sesquiterpene, and Diterpene Synthase Genes in Response to Wounding
Plant Physiology, April 1, 1998; 116(4): 1497 - 1504.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
C. L. Steele, J. Crock, J. Bohlmann, and R. Croteau
Sesquiterpene Synthases from Grand Fir (Abies grandis). COMPARISON OF CONSTITUTIVE AND WOUND-INDUCED ACTIVITIES, AND cDNA ISOLATION, CHARACTERIZATION, AND BACTERIAL EXPRESSION OF delta -SELINENE SYNTHASE AND gamma -HUMULENE SYNTHASE
J. Biol. Chem., January 23, 1998; 273(4): 2078 - 2089.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Bohlmann, C. L. Steele, and R. Croteau
Monoterpene Synthases from Grand Fir (Abies grandis). cDNA ISOLATION, CHARACTERIZATION, AND FUNCTIONAL EXPRESSION OF MYRCENE SYNTHASE, (-)-(4S)-LIMONENE SYNTHASE, AND (-)-(1S,5S)-PINENE SYNTHASE
J. Biol. Chem., August 29, 1997; 272(35): 21784 - 21792.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. Shen, Z. Zheng, and H. K. Dooner
A maize sesquiterpene cyclase gene induced by insect herbivory and volicitin: Characterization of wild-type and mutant alleles
PNAS, December 19, 2000; 97(26): 14807 - 14812.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1994 by the American Society of Plant Biologists