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PLANT PHYSIOLOGY , Vol 106, Issue 4 1359-1365, Copyright © 1994 by American Society of Plant Biologists
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ENVIRONMENTAL AND STRESS PHYSIOLOGY |
A Salt-Induced 60-Kilodalton Plasma Membrane Protein Plays a Potential Role in the Extreme Halotolerance of the Alga Dunaliella
M. Fisher, U. Pick and A. Zamir
Biochemistry Department, Weizmann Institute of Science, Rehovot 76100, Israel
The halotolerant alga Dunaliella salina grows in saline conditions as
varied as 0.5 and 5 M NaCl, maintaining throughout this range a low
intracellular ion concentration. To discover factors potentially involved
in ionic homeostasis, we grew cells in media with different salinities or
osmolarities and compared their protein profiles. The comparisons indicated
that the amount of a 60-kD protein, p60, greatly increased with an increase
in salinity and was moderately enhanced when NaCl was substituted with
iso-osmotic glycerol. Cells transferred from low to high NaCl or from high
glycerol to iso-osmotic NaCl media transiently ceased to grow, and
resumption of growth coincided approximately with an increase in p60. The
protein, extracted from a plasma membrane fraction, was purified to
homogeneity. Anti-p60 antibodies cross-reacted with a 60-kD protein in
Dunaliella bardawil. Immunoelectron microscopy of D. salina cell sections
indicated that p60 was exclusively located in the plasma membrane. Its
induction by salt, the correlation between its accumulation and growth
resumption in high concentrations of salt, and its plasma membrane
localization suggest the possibility that p60 could play a role in ionic
homeostasis in conditions of high salinity, although different types of
function could also be considered.
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