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PLANT PHYSIOLOGY , Vol 106, Issue 4 1367-1372, Copyright © 1994 by American Society of Plant Biologists
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METABOLISM AND ENZYMOLOGY |
The Metabolism of Gibberellin A20 to Gibberellin A1 by Tall and Dwarf Mutants of Oryza sativa and Arabidopsis thaliana
M. Kobayashi, P. Gaskin, C. R. Spray, B. O. Phinney and J. MacMillan
Department of Biology, University of California, Los Angeles, California 90024-1606 (M.K., C.R.S., B.O.P.)
The purpose of this study was to demonstrate the metabolism of gibberellin
A20 (GA20) to gibberellin A1 (GA1) by tall and mutant shoots of rice (Oryza
sativa L.) and Arabidopsis thaliana (L.) Heynh. The data show that the tall
and dx mutant of rice and the tall and ga5 mutant of Arabidopsis metabolize
GA20 to GA1. The data also show that the dy mutant of rice and the ga4
mutant of Arabidopsis block the metabolism of GA20 to GA1. [17-13C,3H]GA20
was fed to tall and the dwarf mutants, dx and dy, of rice and tall and the
dwarf mutants, ga5 and ga4, of Arabidopsis. The metabolites were analyzed
by high-performance liquid chromatography and full-scan gas
chromatography-mass spectrometry together with Kovats retention index data.
For rice, the metabolite [13C]GA, was identified from tall and dx
seedlings; [13C]GA1 was not identified from the dy seedlings. [13C]GA29 was
identified from tall, dx, and dy seedlings. For Arabidopsis, the metabolite
[13C]GA1 was identified from tall, ga5, and ga4 plants. The amount of
[13C]GA1 from ga4 plants was less than 15% of that obtained from tall and
ga5 plants. [13C]GA29 was identified from tall, ga5, and ga4 plants.
[13C]GA5 and [13C]GA3 were not identified from any of the six types of
plant material.
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