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PLANT PHYSIOLOGY , Vol 106, Issue 4 1443-1451, Copyright © 1994 by American Society of Plant Biologists
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METABOLISM AND ENZYMOLOGY |
A Mutant of Arabidopsis with Increased Levels of Stearic Acid
J. Lightner, J. Wu and J. Browse
Institute of Biological Chemistry, Washington State University, Pullman, Washington 99164-6340
A mutation at the fab2 locus of Arabidopsis caused increased levels of
stearate in leaves. The increase in leaf stearate in fab2 varied
developmentally, and the largest increase occurred in young leaves, where
stearate accounted for almost 20% of total leaf fatty acids. The fatty acid
composition of leaf lipids isolated from the fab2 mutant showed increased
stearate in all the major glycerolipids of both the chloroplast and
extrachloroplast membranes. Although the stearate content was increased,
the fab2 mutant still contained abundant amounts of 18:1, 18:2, and 18:3
fatty acids. These results are consistent with the expectations for a
mutation partially affecting the action of the stromal stearoyl-acyl
carrier protein desaturase. Positional analysis indicated that the extra
18:0 is excluded with high specificity from the sn-2 position of both
chloroplast and extrachloroplast glycerolipids. Although stearate content
was increased in all the major leaf membrane lipids, the amount of increase
varied considerably among the different lipids, from a high of 25% of fatty
acids in phosphatidylcholine to a low of 2.9% of fatty acids in
monogalactosyldiacylglycerol.
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