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PLANT PHYSIOLOGY , Vol 107, Issue 3 709-717, Copyright © 1995 by American Society of Plant Biologists
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DEVELOPMENT AND GROWTH REGULATION |
Stability of the Apoproteins of Light-Harvesting Complex I and II during Biogenesis of Thylakoids in the Chlorophyll b-less Barley Mutant Chlorina f2
S. Preiss and J. P. Thornber
Department of Biology, University of California, Los Angeles, California, 90095-1606
Transcription and translation of Lhc (cab) genes have been compared in the
chlorina f2 mutant of barley (Hordeum vulgare) and its wild type to study
the effect of chlorophyll b's absence on the regulation of assembly of the
light-harvesting complexes (LHC). All tested genes were transcribed and the
amount of their respective mRNAs increased rhythmically upon illumination
of etiolated mutant plants. The synthesis of individual LHC apoproteins
also had a rhythmic pattern when total leaf protein extracts were examined,
whereas they increased gradually in the thylakoid. Only some LHC
pigment-proteins present in wild-type thylakoids were found in mature
mutant membranes. Thus, only the 25-kD (type 3) apoprotein of the three
apoproteins of the major LHC IIb complex survived. The amount of the minor
LHC II pigment-proteins was considerably reduced but not to zero.
Photosystem I had some of the two LHC la apoproteins but had little of
those of LHC lb. This was reflected in a shift of the 77-K emission maximum
of whole leaves from 741 to 732 nm. It is concluded that the two largest
LHC IIb and the LHC Ib apoproteins need chlorophyll b for stable
integration into the membrane and that posttranslational regulation plays a
major role in LHC assembly.
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