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PLANT PHYSIOLOGY , Vol 107, Issue 3 915-923, Copyright © 1995 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Cold-Induced Accumulation of hsp90 Transcripts in Brassica napus
P. Krishna, M. Sacco, J. F. Cherutti and S. Hill
Department of Plant Sciences, The University of Western Ontario, 1151 Richmond Street North, London, Ontario, Canada N6A 5B7
Characterization of the expression of hsp90 genes of Brassica napus by
northern blot analysis and immunoblotting showed that the hsp90 mRNA and
protein are present in all B. napus tissues examined, albeit at different
levels. High levels of hsp90 mRNA and protein were found in young and
rapidly dividing tissues such as shoot apices and flower buds, suggesting
that hsp90 may have an important role in plant growth and development. A
significant increase in hsp90 mRNA levels was detected in seedlings exposed
to 5[deg]C. The transcript levels reached a maximum within 1 d of cold
treatment and remained elevated for the entire duration of cold treatment.
The levels of hsp90 mRNA rapidly decreased to the level found in control
plants upon return to 20[deg]C. The cold-induced accumulation of hsp90 mRNA
closely resembles the expression of two previously identified
cold-regulated genes of B. napus. We have also confirmed cold regulation of
hsp90 mRNA in spinach (Spinacea oleracea). Our results suggest a role for
hsp90 in adaptation to cold temperature stress.
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