Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (40)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Suss, K. H.
Right arrow Articles by Adler, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Suss, K. H.
Right arrow Articles by Adler, K.
Agricola
Right arrow Articles by Suss, K. H.
Right arrow Articles by Adler, K.

PLANT PHYSIOLOGY , Vol 107, Issue 4 1387-1397, Copyright © 1995 by American Society of Plant Biologists

BIOCHEMISTRY AND ENZYMOLOGY

In Situ Association of Calvin Cycle Enzymes, Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase, Ferredoxin-NADP+ Reductase, and Nitrite Reductase with Thylakoid and Pyrenoid Membranes of Chlamydomonas reinhardtii Chloroplasts as Revealed by Immunoelectron Microscopy

K. H. Suss, I. Prokhorenko and K. Adler
Department of Molecular Cell Biology, Institute of Plant Genetics and Crop Plant Research, 06466 Gatersleben, Germany (K.-H.S., K.A.)

The in situ localization of the chloroplast enzymes ribulose-1,5-bisphosphate carboxylase (Rubisco), Rubisco activase, ribose-5-phosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, aldolase, nitrite reductase, ferredoxin-NADP+ reductase, and H+-ATP synthase was studied by immunoelectron microscopy in Chlamydomonas reinhardtii. Immunogold labeling revealed that, despite Rubisco in the pyrenoid matrix, Calvin cycle enzymes, Rubisco activase, nitrite reductase, ferredoxin-NADP+ reductase, and H+-ATP synthase are associated predominantly with chloroplast thylakoid membranes and the inner surface of the pyrenoid membrane. This is in accord with previous enzyme localization studies in higher plants (K.H. Suss, C. Arkona, R. Manteuffel, K. Adler [1993] Proc Natl Acad Sci USA 90: 5514-5518). Pyrenoid tubules do not contain these enzymes. The pyrenoid matrix consists of Rubisco but is devoid of the other photosynthetic enzymes investigated. Evidence for the occurrence of two Rubisco forms differing in their spatial localization has also been obtained: Rubisco form I appears to be membrane associated like other Calvin cycle components, whereas Rubisco form II is confined to the pyrenoid matrix. It is proposed that enzyme form I represents an active Rubisco when assembled into Calvin cycle enzyme complexes, whereas Rubisco form II may be part of a CO2-concentrating mechanism. Pyrenoidal Calvin cycle complexes are thought to be highly active in CO2 fixation and important for the synthesis of starch around the pyrenoid.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Uniacke and W. Zerges
Chloroplast protein targeting involves localized translation in Chlamydomonas
PNAS, February 3, 2009; 106(5): 1439 - 1444.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
R. Agarwal, S. Ortleb, J. K. Sainis, and M. Melzer
Immunoelectron Microscopy for Locating Calvin Cycle Enzymes in the Thylakoids of Synechocystis 6803
Mol Plant, January 1, 2009; 2(1): 32 - 42.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. Uniacke and W. Zerges
Stress induces the assembly of RNA granules in the chloroplast of Chlamydomonas reinhardtii
J. Cell Biol., August 25, 2008; 182(4): 641 - 646.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. L. Houtz, R. Magnani, N. R. Nayak, and L. M. A. Dirk
Co- and post-translational modifications in Rubisco: unanswered questions
J. Exp. Bot., May 1, 2008; 59(7): 1635 - 1645.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. Uniacke and W. Zerges
Photosystem II Assembly and Repair Are Differentially Localized in Chlamydomonas
PLANT CELL, November 1, 2007; 19(11): 3640 - 3654.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
P. Deschamps, I. Haferkamp, D. Dauvillee, S. Haebel, M. Steup, A. Buleon, J.-L. Putaux, C. Colleoni, C. d'Hulst, C. Plancke, et al.
Nature of the Periplastidial Pathway of Starch Synthesis in the Cryptophyte Guillardia theta
Eukaryot. Cell, June 1, 2006; 5(6): 954 - 963.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
I. Haferkamp, P. Deschamps, M. Ast, W. Jeblick, U. Maier, S. Ball, and H. E. Neuhaus
Molecular and Biochemical Analysis of Periplastidial Starch Metabolism in the Cryptophyte Guillardia theta
Eukaryot. Cell, June 1, 2006; 5(6): 964 - 971.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Rachmilevitch, A. B. Cousins, and A. J. Bloom
Nitrate assimilation in plant shoots depends on photorespiration
PNAS, August 3, 2004; 101(31): 11506 - 11510.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Graciet, S. Lebreton, and B. Gontero
Emergence of new regulatory mechanisms in the Benson-Calvin pathway via protein-protein interactions: a glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase complex
J. Exp. Bot., June 1, 2004; 55(400): 1245 - 1254.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Lebreton, E. Graciet, and B. Gontero
Modulation, via Protein-Protein Interactions, of Glyceraldehyde-3-phosphate Dehydrogenase Activity through Redox Phosphoribulokinase Regulation
J. Biol. Chem., March 28, 2003; 278(14): 12078 - 12084.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Mouche, B. Gontero, I. Callebaut, J.-P. Mornon, and N. Boisset
Striking Conformational Change Suspected within the Phosphoribulokinase Dimer Induced by Interaction with GAPDH
J. Biol. Chem., February 15, 2002; 277(8): 6743 - 6749.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Avilan, S. Lebreton, and B. Gontero
Thioredoxin Activation of Phosphoribulokinase in a Bi-enzyme Complex from Chlamydomonas reinhardtii Chloroplasts
J. Biol. Chem., March 24, 2000; 275(13): 9447 - 9451.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Lebreton and B. Gontero
Memory and Imprinting in Multienzyme Complexes. EVIDENCE FOR INFORMATION TRANSFER FROM GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE TO PHOSPHORIBULOKINASE UNDER REDUCED STATE IN CHLAMYDOMONAS REINHARDTII
J. Biol. Chem., July 23, 1999; 274(30): 20879 - 20884.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Eichelmann and A. Laisk
Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Content, Assimilatory Charge, and Mesophyll Conductance in Leaves
Plant Physiology, January 1, 1999; 119(1): 179 - 190.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
O. N. Borkhsenious, C. B. Mason, and J. V. Moroney
The Intracellular Localization of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in Chlamydomonas reinhardtii
Plant Physiology, April 1, 1998; 116(4): 1585 - 1591.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. J. Bloom, D. R. Smart, D. T. Nguyen, and P. S. Searles
Nitrogen assimilation and growth of wheat under elevated carbon dioxide
PNAS, February 5, 2002; 99(3): 1730 - 1735.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1995 by the American Society of Plant Biologists