Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (21)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Peterson, R. B.
Right arrow Articles by Aylor, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Peterson, R. B.
Right arrow Articles by Aylor, D. E.
Agricola
Right arrow Articles by Peterson, R. B.
Right arrow Articles by Aylor, D. E.

PLANT PHYSIOLOGY , Vol 108, Issue 1 163-171, Copyright © 1995 by American Society of Plant Biologists

PLANT-MICROBE AND PLANT-INSECT INTERACTIONS

Chlorophyll Fluorescence Induction in Leaves of Phaseolus vulgaris Infected with Bean Rust (Uromyces appendiculatus)

R. B. Peterson and D. E. Aylor
Department of Biochemistry and Genetics (R.B.P.) and Department of Plant Pathology and Ecology (D.E.A.), The Connecticut Agricultural Experiment Station, 123 Huntington Street, New Haven, Connecticut 06511

To our knowledge, this report describes the first application of video imaging of Chl fluorescence to the study of light utilization in photosystem II of attached leaves of Phaseolus vulgaris infected with the obligate biotrophic fungus Uromyces appendiculatus (race 38). The video-based detection system produced a spatially resolved, quantifiable signal that was highly specific for chlorophyll fluorescence. Video images of spatial variation in the initial stage of the fluorescence induction (dark-light) transient revealed discreet regions of intense emission coinciding with centers of subsequent lesion development and accompanying chlorosis. Incipient lesions were visible by this procedure 3 d following inoculation, fully 3 to 4 d prior to visible symptoms. Fluorescence emission patterns in infected areas during the induction transient were heterogeneous with radial distance from the point of invasion and varied with the length of the time delay between re-illumination and image capture. During later ([greater than or equal to]1 min) stages of the induction transient, fluorescence emission in incipient lesions was quenched compared to surrounding tissue. These essential features of the induction transient observed in video images were also noted when individual lesions were examined using pulse modulation fluorimetry.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1995 by the American Society of Plant Biologists