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PLANT PHYSIOLOGY , Vol 108, Issue 1 261-268, Copyright © 1995 by American Society of Plant Biologists

CELL BIOLOGY AND SIGNAL TRANSDUCTION

Correct Processing of the Kiwifruit Protease Actinidin in Transgenic Tobacco Requires the Presence of the C-Terminal Propeptide

W. Paul, J. Amiss, R. Try, U. Praekelt, R. Scott and H. Smith
Department of Botany, Leicester University, University Road, Leicester LE1 7RH, United Kingdom

A 35S cauliflower mosaic virus promoter and a tapetum-specific promoter were used to direct the synthesis in tobacco of preproactinidin and a derivative that lacked a C-terminal extension. Preproactinidin was processed into a form that migrated identically on protein gels with mature actinidin extracted from kiwifruit. This protein was proteolytically active in vitro, and high-level accumulation of this protein appeared to be detrimental to plant growth. Plants expressing an actinidin cDNA construct that lacked the sequence encoding the C-terminal propeptide were phenotypically normal but accumulated N-proactinidin, which was proteolytically active in vitro but did not self-cleave to mature actinidin. In transgenic tobacco, the C-terminal extension of actinidin is therefore required for correct processing.


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M. P. Does, P. M. Houterman, H. L. Dekker, and B. J.C. Cornelissen
Processing, Targeting, and Antifungal Activity of Stinging Nettle Agglutinin in Transgenic Tobacco
Plant Physiology, June 1, 1999; 120(2): 421 - 432.
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Copyright © 1995 by the American Society of Plant Biologists