Plant Physiol.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (84)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Katavic, V.
Right arrow Articles by Kunst, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Katavic, V.
Right arrow Articles by Kunst, L.
Agricola
Right arrow Articles by Katavic, V.
Right arrow Articles by Kunst, L.

PLANT PHYSIOLOGY , Vol 108, Issue 1 399-409, Copyright © 1995 by American Society of Plant Biologists

BIOCHEMISTRY AND ENZYMOLOGY

Alteration of Seed Fatty Acid Composition by an Ethyl Methanesulfonate-Induced Mutation in Arabidopsis thaliana Affecting Diacylglycerol Acyltransferase Activity

V. Katavic, D. W. Reed, D. C. Taylor, E. M. Giblin, D. L. Barton, J. Zou, S. L. MacKenzie, P. S. Covello and L. Kunst
National Research Council of Canada, Plant Biotechnology Institute, 110 Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N 0W9

In characterizing the enzymes involved in the formation of very long-chain fatty acids (VLCFAs) in the Brassicaceae, we have generated a series of mutants of Arabidopsis thaliana that have reduced VLCFA content. Here we report the characterization of a seed lipid mutant, AS11, which, in comparison to wild type (WT), has reduced levels of 20:1 and 18:1 and accumulates 18:3 as the major fatty acid in triacylglycerols. Proportions of 18:2 remain similar to WT. Genetic analyses indicate that the fatty acid phenotype is caused by a semidominant mutation in a single nuclear gene, designated TAG1, located on chromosome 2. Biochemical analyses have shown that the AS11 phenotype is not due to a deficiency in the capacity to elongate 18:1 or to an increase in the relative [delta]15 or [delta]12 desaturase activities. Indeed, the ratio of desaturase/elongase activities measured in vitro is virtually identical in developing WT and AS11 seed homogenates. Rather, the fatty acid phenotype of AS11 is the result of reduced diacylglycerol acyltransferase activity throughout development, such that triacylglycerol biosynthesis is reduced. This leads to a reduction in 20:1 biosynthesis during seed development, leaving more 18:1 available for desaturation. Thus, we have demonstrated that changes to triacylglycerol biosynthesis can result in dramatic changes in fatty acid composition and, in particular, in the accumulation of VLCFAs in seed storage lipids.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
M. Chen, B. P. Mooney, M. Hajduch, T. Joshi, M. Zhou, D. Xu, and J. J. Thelen
System Analysis of an Arabidopsis Mutant Altered in de Novo Fatty Acid Synthesis Reveals Diverse Changes in Seed Composition and Metabolism
Plant Physiology, May 1, 2009; 150(1): 27 - 41.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
P. D. Bates, T. P. Durrett, J. B. Ohlrogge, and M. Pollard
Analysis of Acyl Fluxes through Multiple Pathways of Triacylglycerol Synthesis in Developing Soybean Embryos
Plant Physiology, May 1, 2009; 150(1): 55 - 72.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. J. Weselake, S. Shah, M. Tang, P. A. Quant, C. L. Snyder, T. L. Furukawa-Stoffer, W. Zhu, D. C. Taylor, J. Zou, A. Kumar, et al.
Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content
J. Exp. Bot., October 1, 2008; 59(13): 3543 - 3549.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
A. F. Alvarez, H. M. Alvarez, R. Kalscheuer, M. Waltermann, and A. Steinbuchel
Cloning and characterization of a gene involved in triacylglycerol biosynthesis and identification of additional homologous genes in the oleaginous bacterium Rhodococcus opacus PD630
Microbiology, August 1, 2008; 154(8): 2327 - 2335.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
J. J. Wassom, V. Mikkelineni, M. O. Bohn, and T. R. Rocheford
QTL for Fatty Acid Composition of Maize Kernel Oil in Illinois High Oil x B73 Backcross-Derived Lines
Crop Sci., January 16, 2008; 48(1): 69 - 78.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Fait, R. Angelovici, H. Less, I. Ohad, E. Urbanczyk-Wochniak, A. R. Fernie, and G. Galili
Arabidopsis Seed Development and Germination Is Associated with Temporally Distinct Metabolic Switches
Plant Physiology, November 1, 2006; 142(3): 839 - 854.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. M. Shockey, S. K. Gidda, D. C. Chapital, J.-C. Kuan, P. K. Dhanoa, J. M. Bland, S. J. Rothstein, R. T. Mullen, and J. M. Dyer
Tung Tree DGAT1 and DGAT2 Have Nonredundant Functions in Triacylglycerol Biosynthesis and Are Localized to Different Subdomains of the Endoplasmic Reticulum
PLANT CELL, September 1, 2006; 18(9): 2294 - 2313.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Penfield, E. L. Rylott, A. D. Gilday, S. Graham, T. R. Larson, and I. A. Graham
Reserve Mobilization in the Arabidopsis Endosperm Fuels Hypocotyl Elongation in the Dark, Is Independent of Abscisic Acid, and Requires PHOSPHOENOLPYRUVATE CARBOXYKINASE1
PLANT CELL, October 1, 2004; 16(10): 2705 - 2718.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. H. Hobbs, J. E. Flintham, and M. J. Hills
Genetic Control of Storage Oil Synthesis in Seeds of Arabidopsis
Plant Physiology, October 1, 2004; 136(2): 3341 - 3349.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Moire, E. Rezzonico, S. Goepfert, and Y. Poirier
Impact of Unusual Fatty Acid Synthesis on Futile Cycling through {beta}-Oxidation and on Gene Expression in Transgenic Plants
Plant Physiology, January 1, 2004; 134(1): 432 - 442.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
Z. Zheng, Q. Xia, M. Dauk, W. Shen, G. Selvaraj, and J. Zou
Arabidopsis AtGPAT1, a Member of the Membrane-Bound Glycerol-3-Phosphate Acyltransferase Gene Family, Is Essential for Tapetum Differentiation and Male Fertility
PLANT CELL, August 1, 2003; 15(8): 1872 - 1887.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E.-F. Marillia, B. J. Micallef, M. Micallef, A. Weninger, K. K. Pedersen, J. Zou, and D. C. Taylor
Biochemical and physiological studies of Arabidopsis thaliana transgenic lines with repressed expression of the mitochondrial pyruvate dehydrogenase kinase1
J. Exp. Bot., January 2, 2003; 54(381): 259 - 270.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. T. Kaup, C. D. Froese, and J. E. Thompson
A Role for Diacylglycerol Acyltransferase during Leaf Senescence
Plant Physiology, August 1, 2002; 129(4): 1616 - 1626.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Lu and M. J. Hills
Arabidopsis Mutants Deficient in Diacylglycerol Acyltransferase Display Increased Sensitivity to Abscisic Acid, Sugars, and Osmotic Stress during Germination and Seedling Development
Plant Physiology, July 1, 2002; 129(3): 1352 - 1358.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. D. Lardizabal, J. T. Mai, N. W. Wagner, A. Wyrick, T. Voelker, and D. J. Hawkins
DGAT2 Is a New Diacylglycerol Acyltransferase Gene Family. PURIFICATION, CLONING, AND EXPRESSION IN INSECT CELLS OF TWO POLYPEPTIDES FROM MORTIERELLA RAMANNIANA WITH DIACYLGLYCEROL ACYLTRANSFERASE ACTIVITY
J. Biol. Chem., October 12, 2001; 276(42): 38862 - 38869.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Jako, A. Kumar, Y. Wei, J. Zou, D. L. Barton, E. M. Giblin, P. S. Covello, and D. C. Taylor
Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight
Plant Physiology, June 1, 2001; 126(2): 861 - 874.
[Abstract] [Full Text] [PDF]

Home page
 Crop Sci.Home page
V. Katavic, W. Friesen, D. L. Barton, K. K. Gossen, E.M. Giblin, T. Luciw, J. An, J. Zou, S. L. MacKenzie, W. A. Keller, et al.
Improving Erucic Acid Content in Rapeseed through Biotechnology: What Can the Arabidopsis FAE1 and the Yeast SLC1-1 Genes Contribute?,
Crop Sci., May 1, 2001; 41(3): 739 - 747.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Girke, J. Todd, S. Ruuska, J. White, C. Benning, and J. Ohlrogge
Microarray Analysis of Developing Arabidopsis Seeds
Plant Physiology, December 1, 2000; 124(4): 1570 - 1581.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
Y. Poirier, G. Ventre, and D. Caldelari
Increased Flow of Fatty Acids toward beta -Oxidation in Developing Seeds of Arabidopsis Deficient in Diacylglycerol Acyltransferase Activity or Synthesizing Medium-Chain-Length Fatty Acids
Plant Physiology, December 1, 1999; 121(4): 1359 - 1366.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
N. Focks and C. Benning
wrinkled1: A Novel, Low-Seed-Oil Mutant of Arabidopsis with a Deficiency in the Seed-Specific Regulation of Carbohydrate Metabolism
Plant Physiology, September 1, 1998; 118(1): 91 - 101.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1995 by the American Society of Plant Biologists