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PLANT PHYSIOLOGY , Vol 108, Issue 1 99-103, Copyright © 1995 by American Society of Plant Biologists
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BIOCHEMISTRY AND ENZYMOLOGY |
Subcellular Compartmentation of the 4-Aminobutyrate Shunt in Protoplasts from Developing Soybean Cotyledons
K. E. Breitkreuz and B. J. Shelp
Department of Horticultural Science and Interdepartmental Plant Physiology Program, University of Guelph, Guelph, Ontario, Canada N1G 2W1
The subcellular localization of enzymes involved in the 4-ami-nobutyrate
shunt was investigated in protoplasts prepared from developing soybean
[Glycine max (L.) Merrill cv Maple Arrow] cotyledons. Protoplast lysate was
fractionated by differential and continuous Percoll-gradient centrifugation
to separate organelle fractions. Glutamate decarboxylase (EC 4.1.1.15) was
found exclusively in the cytosol, whereas 4-aminobutyrate:pyruvate
transami-nase (EC 2.6.1.19) and succinic semialdehyde dehydrogenase (EC
1.2.1.16) were associated exclusively with the mitochondrial fractions.
Mitochondrial fractions also catabolized [U-14C]4-aminobu-tyrate to labeled
succinate.
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