PLANT PHYSIOLOGY , Vol 108, Issue 2 469-474, Copyright © 1995 by American Society of Plant Biologists
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DEVELOPMENT AND GROWTH REGULATION |
Mitochondria Increase Three-Fold and Mitochondrial Proteins and Lipid Change Dramatically in Postmeristematic Cells in Young Wheat Leaves Grown in Elevated CO2
E. J. Robertson, M. Williams, J. L. Harwood, J. G. Lindsay, C. J. Leaver and R. M. Leech
Department of Biology, University of York, Heslington, York, YO1 5DD (E.J.R., R.M.L.)
A dramatic stimulation in mitochondrial biogenesis during the very early
stages of leaf development was observed in young wheat plants (Triticum
aestivum cv Hereward) grown in elevated CO2 (650 [mu]L L-1). An almost
3-fold increase in the number of mitochondria was observed in the very
young leaf cells at the base of the first leaf of a 7-d-old wheat plant. In
the same cells large increases in the accumulation of a mitochondrial
chaperonin protein and the mitochondrial 2-oxoglutarate dehydrogenase
complex and pyruvate dehydrogenase complex were detected by immunolabeling.
Furthermore, the basal segment also shows a large increase in the rate of
radiolabeling of diphosphatidylglycerol, a lipid confined to the inner
mitochondrial membrane. This dramatic response in very young leaf cells to
elevated CO2 suggests that the numerous documented positive effects of
elevated CO2 on wheat leaf development are initiated as early as 12 h
postmitosis.
