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PLANT PHYSIOLOGY , Vol 108, Issue 2 589-595, Copyright © 1995 by American Society of Plant Biologists
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WHOLE PLANT, ENVIRONMENTAL, AND STRESS PHYSIOLOGY |
Nucleotide Levels Do Not Critically Determine Survival of Maize Root Tips Acclimated to a Low-Oxygen Environment
J. H. Xia, P. Saglio and JKM. Roberts
Department of Biochemistry, University of California, Riverside, California 92521 (J-H.X., J.K.M.R.)
We tested the hypothesis that ATP levels and energy charge determine the
resistance of maize (Zea mays) root tips to anoxia. We focused on root tips
of whole maize seedlings that had been acclimated to low O2 by exposure to
an atmosphere of 3% (v/v) O2 in N2. Acclimated anoxic root tips
characteristically have higher ATP levels and energy charge and survive
longer under anoxia than nonacclimated tips. We poisoned intact, acclimated
root tips with either fluoride or mannose, causing decreases in ATP and
energy charge to values similar to or, in most cases, below those found in
nonacclimated anoxic tips. With the exception of the highest fluoride
concentration used, the poisoned, acclimated tips remained much more
tolerant of anoxia than nonacclimated root tips. We conclude that high ATP
and energy charge are not components critical for the survival of
acclimated root tips during anoxia. The reduced nucleotide status in
poisoned, acclimated root tips had little effect on cytoplasmic pH
regulation during anoxia. This result indicates that in anoxic, acclimated
root tips either cytoplasmic pH regulation is not dominated by
ATP-dependent processes or these processes can continue in vivo largely
independently of any changes in ATP levels in the physiological range. The
role of glycolytic flux in survival under anoxia is discussed.
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