|
PLANT PHYSIOLOGY , Vol 108, Issue 2 609-614, Copyright © 1995 by American Society of Plant Biologists
|
BIOCHEMISTRY AND ENZYMOLOGY |
Enzymatic Evidence for a Complete Oxidative Pentose Phosphate Pathway in Chloroplasts and an Incomplete Pathway in the Cytosol of Spinach Leaves
C. Schnarrenberger, A. Flechner and W. Martin
Institut fur Pflanzenphysiologie und Mikrobiologie, Freie Universitat Berlin, Konigin-Luise-Strasse 12-16a, D-14195 Berlin, Germany (C.S., A.F.)
The intracellular localization of transaldolase, transketolase,
ribose-5-phosphate isomerase, and ribulose-5-phosphate epimerase was
reexamined in spinach (Spinacia oleracea L.) leaves. We found highly
predominant if not exclusive localization of these enzyme activities in
chloroplasts isolated by isopyknic centrifugation in sucrose gradients.
Glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase,
glucose phosphate isomerase, and triose phosphate isomerase activity was
present in the chloroplast fraction but showed additional activity in the
cytosol (supernatant) fraction attributable to the cytosol-specific
isoforms known to exist for these enzymes. Anion-exchange chromatography of
proteins of crude extracts on diethylaminoethyl-Fractogel revealed only a
single enzyme each for transaldolase, transketolase, ribose-5-phosphate
isomerase, and ribulose-5-phosphate epimerase. The data indicate that
chloroplasts of spinach leaf cells possess the complete complement of
enzymes of the oxidative pentose phosphate path-way (OPPP), whereas the
cytosol contains only the first two reactions, contrary to the widely held
view that plants generally possess a cytosolic OPPP capable of cyclic
function. The chloroplast enzymes transketolase, ribose-5-phosphate
isomerase, and ribulose-5-phosphate epimerase appear to be amphibolic for
the Calvin cycle and OPPP.
This article has been cited by other articles:
|
|
|
|
 
Y. Xiong, C. DeFraia, D. Williams, X. Zhang, and Z. Mou
Characterization of Arabidopsis 6-Phosphogluconolactonase T-DNA Insertion Mutants Reveals an Essential Role for the Oxidative Section of the Plastidic Pentose Phosphate Pathway in Plant Growth and Development
Plant Cell Physiol.,
July 1, 2009;
50(7):
1277 - 1291.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Scharte, H. Schon, Z. Tjaden, E. Weis, and A. von Schaewen
Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants
PNAS,
May 12, 2009;
106(19):
8061 - 8066.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. G. Bowsher, A. E. Lacey, G. T. Hanke, D. T. Clarkson, L. R. Saker, I. Stulen, and M. J. Emes
The effect of Glc6P uptake and its subsequent oxidation within pea root plastids on nitrite reduction and glutamate synthesis
J. Exp. Bot.,
March 1, 2007;
58(5):
1109 - 1118.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Hemmerlin, D. Tritsch, M. Hartmann, K. Pacaud, J.-F. Hoeffler, A. van Dorsselaer, M. Rohmer, and T. J. Bach
A Cytosolic Arabidopsis D-Xylulose Kinase Catalyzes the Phosphorylation of 1-Deoxy-D-Xylulose into a Precursor of the Plastidial Isoprenoid Pathway
Plant Physiology,
October 1, 2006;
142(2):
441 - 457.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Petersen, R. Teich, B. Becker, R. Cerff, and H. Brinkmann
The GapA/B Gene Duplication Marks the Origin of Streptophyta (Charophytes and Land Plants)
Mol. Biol. Evol.,
June 1, 2006;
23(6):
1109 - 1118.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. K. Michels, N. Wedel, and P. G. Kroth
Diatom Plastids Possess a Phosphoribulokinase with an Altered Regulation and No Oxidative Pentose Phosphate Pathway
Plant Physiology,
March 1, 2005;
137(3):
911 - 920.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Hutchings, S. Rawsthorne, and M. J. Emes
Fatty acid synthesis and the oxidative pentose phosphate pathway in developing embryos of oilseed rape (Brassica napus L.)
J. Exp. Bot.,
February 1, 2005;
56(412):
577 - 585.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Hauschild and A. von Schaewen
Differential Regulation of Glucose-6-Phosphate Dehydrogenase Isoenzyme Activities in Potato
Plant Physiology,
September 1, 2003;
133(1):
47 - 62.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Schwender, J. B. Ohlrogge, and Y. Shachar-Hill
A Flux Model of Glycolysis and the Oxidative Pentosephosphate Pathway in Developing Brassica napus Embryos
J. Biol. Chem.,
August 8, 2003;
278(32):
29442 - 29453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gerhardt, S. Echt, M. Busch, J. Freigang, G. Auerbach, G. Bader, W. F. Martin, A. Bacher, R. Huber, and M. Fischer
Structure and Properties of an Engineered Transketolase from Maize
Plant Physiology,
August 1, 2003;
132(4):
1941 - 1949.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. del Rio, F. J. Corpas, L. M. Sandalio, J. M. Palma, M. Gomez, and J. B. Barroso
Reactive oxygen species, antioxidant systems and nitric oxide in peroxisomes
J. Exp. Bot.,
May 15, 2002;
53(372):
1255 - 1272.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Eicks, V. Maurino, S. Knappe, U.-I. Flugge, and K. Fischer
The Plastidic Pentose Phosphate Translocator Represents a Link between the Cytosolic and the Plastidic Pentose Phosphate Pathways in Plants
Plant Physiology,
February 1, 2002;
128(2):
512 - 522.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Henkes, U. Sonnewald, R. Badur, R. Flachmann, and M. Stitt
A Small Decrease of Plastid Transketolase Activity in Antisense Tobacco Transformants Has Dramatic Effects on Photosynthesis and Phenylpropanoid Metabolism
PLANT CELL,
March 1, 2001;
13(3):
535 - 551.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Kopriva, A. Koprivova, and K.-H. Suss
Identification, Cloning, and Properties of Cytosolic D-Ribulose-5-phosphate 3-Epimerase from Higher Plants
J. Biol. Chem.,
January 14, 2000;
275(2):
1294 - 1299.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-R. Chen, F. C. Hartman, T.-Y. S. Lu, and F. W. Larimer
D-Ribulose-5-Phosphate 3-Epimerase: Cloning and Heterologous Expression of the Spinach Gene, and Purification and Characterization of the Recombinant Enzyme
Plant Physiology,
September 1, 1998;
118(1):
199 - 207.
[Abstract]
[Full Text]
|
|
|
|
|
