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PLANT PHYSIOLOGY , Vol 108, Issue 2 651-657, Copyright © 1995 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Differential Expression of Two 1-Aminocyclopropane-1-Carboxylic Acid Oxidase Genes in Broccoli after Harvest
B. J. Pogson, C. G. Downs and K. M. Davies
Division of Horticulture, Commonwealth Scientific and Industrial Research Organization, P.O. Box 52, Nth Ryde 2113, New South Wales, Australia (B.J.P.)
Broccoli (Brassica oleracea L.) floral tissues rapidly differentiate and
grow before harvest and then senesce rapidly after harvest. Associated with
this postharvest deterioration is an increase in ethylene production by
florets. Two cDNA clones having high nucleotide identity to sequences
encoding 1-amino-cyclopropane-1-carboxylic acid (ACC) oxidase were isolated
from senescing florets. The cDNAs, ACC Ox1 and ACC Ox2, apparently encode
mRNAs from different genes. ACC Ox1 transcripts were found at low levels in
whole florets at the time of harvest and increased markedly in abundance
after harvest. ACC Ox1 transcript abundance also increased in sepals after
harvest and in excised yellowing leaves. Transcripts corresponding to ACC
Ox2 were found exclusively within the reproductive structures. These ACC
Ox2 transcripts were absent at harvest but started to increase in abundance
within 2 h of harvest and then accumulated to high levels. Hormone
treatment did not alter the abundance of ACC Ox1 transcripts, whereas ACC
Ox2 transcripts increased in abundance after treatment with abscisic acid
and propylene. Wounding did not affect the levels of ACC Ox1 or Ox2
transcripts after harvest. At harvest, individual broccoli florets were
closed and remained unpollinated. We propose a model whereby the rapid
increase in ACC Ox1 and Ox2 transcript abundance after harvest contributes
to increased ethylene production by florets. This ethylene may regulate
aspects of postharvest senescence, in particular chlorophyll loss.
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