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PLANT PHYSIOLOGY , Vol 108, Issue 2 795-803, Copyright © 1995 by American Society of Plant Biologists

WHOLE PLANT, ENVIRONMENTAL, AND STRESS PHYSIOLOGY

Expression of a Low-Temperature-Induced Protein in Brassica napus

J. G. Boothe, M. D. de Beus and A. M. Johnson-Flanagan
Department of Agriculture, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5

BN28 is a low-temperature-induced, boiling-soluble protein in Brassica napus. We used antibodies raised against a recombinant BN28 to examine the expression of this protein in cold-acclimating plants and to investigate its relationship to plant freezing tolerance. Changes in the steady-state levels of BN28 protein appear to lag several days behind those of the mRNA. BN28 is first detected on immunoblots after approximately 8 d of exposure to low temperature, and thereafter levels remain stable while plants are maintained at 4[deg]C. Radiolabeling studies indicate that BN28 is synthesized at a relatively low rate. A decline in protein levels is observed soon after returning plants to control temperatures, and little or no protein can be detected after 7 d of deacclimation. The disappearance of the protein precedes a loss in freezing tolerance, suggesting that BN28 is not involved in maintaining plasma membrane integrity. Expression of BN28 is observed primarily in leaves and appears to be low-temperature specific. Quantitative analysis indicated that BN28 accumulates to approximately 82.7 pmol mg-1 total protein in cold-acclimated leaves. This concentration is similar to that reported for two group 2 late-embryogenesis-abundant-like proteins.




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Copyright © 1995 by the American Society of Plant Biologists