PLANT PHYSIOLOGY , Vol 108, Issue 4 1395-1404, Copyright © 1995 by American Society of Plant Biologists

GENE REGULATION AND MOLECULAR GENETICS

Expression of Two Related Vacuolar H+-ATPase 16-Kilodalton Proteolipid Genes Is Differentially Regulated in a Tissue-Specific Manner

M. P. Hasenfratz, C. L. Tsou and T. A. Wilkins
Department of Agronomy and Range Science, University of California, Davis, California 95616-8515

The 16-kD proteolipid subunit is the principal integral membrane protein of the vaculor H+-ATPase (V-ATPase) complex that forms the proton channel responsible for translocating protons across lipid bilayers. Two degenerate synthetic oligonucleotides, COT11 and COT12, corresponding to highly conserved transmembrane domains in all 16-kD subunits sequenced so far, were used to amplify a partial cDNA of the V-ATPase proteolipid subunit from cotton (Gossypium hirsutum L.) by polymerase chain reaction (PCR). These PCR products were used to isolate two full-length cDNAs from a -3 d postanthesis cotton ovule library. Both clones, CVA16.2 and CVA16.4, consisting of 816 and 895 bp, respectively, encode the 16-kD proteolipid subunit of the V-ATPase. At the nucleotide level, the complete sequences of the two clones show 73.5% identity, but share about 95% identity within the coding region, although the two polypeptides differ by only one amino acid. Comparison of deduced amino acid sequences of the proteolipid subunits revealed that the four transmembrane domains and the two cytosolic extramembrane domains are highly conserved in all eukaryotes. Southern blot analysis of cotton genomic DNA showed that these clones belong to small gene families in related diploid and allotetraploid species. Northern blot analysis suggested that the three major V-ATPase subunits (69, 60, and 16 kD) are coordinately regulated, in part, at the transcriptional level. RNA analysis and reverse-transcription PCR established that 16-kD proteolipid transcripts differentially accumulate in different tissues and increase dramatically in tissues undergoing rapid expansion, particularly in anthers, ovules, and petals. The CVA16.4 proteolipid transcript is the most prevalent of the two proteolipid messages in expanding ovules harvested 10 d postanthesis. In contrast, the two proteolipid mRNAs accumulate to similar levels in developing petals.

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