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PLANT PHYSIOLOGY , Vol 109, Issue 1 319-326, Copyright © 1995 by American Society of Plant Biologists
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WHOLE PLANT, ENVIRONMENTAL, AND STRESS PHYSIOLOGY |
Kinetics of NO3- Influx in Spruce
H. J. Kronzucker, M. Y. Siddiqi and ADM. Glass
Department of Botany, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4
Influxes of 13NO3- across the root plasmalemma were measured in intact
seedlings of Picea glauca (Moench) Voss. Three kinetically distinct uptake
systems for NO3- were identified. In seedlings not previously exposed to
external NO3-, a single Michaelis-Menten-type constitutive high-affinity
transport system (CHATS) operated in a 2.5 to 500 [mu]M range of external
NO3- [NO3-]o. The Vmax of this system was 0.1 [mu]mol g-1 h-1, and the Km
was approximately 15 [mu]M. Following exposure to NO3- for 3 d, this CHATS
activity was increased approximately 3-fold, with no change of Km. In
addition, a NO3--inducible high-affinity system became apparent with a Km
of approximately 100[mu]M. The combined Vmax for these discrete saturable
components was 0.7 [mu]mol g-1 h-1. In both uninduced and induced plants a
linear low-affinity system, additive to CHATS and an NO3--inducible
high-affinity system, operated at [NO3-]o [greater than or equal to] 1 mM.
The time taken to achieve maximal rates of uptake (full induction) was 2 d
from 1.5 mM [NO3-]o and 3 d from 200 [mu]M [NO3-]o.
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