Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by de Castro, R. D.
Right arrow Articles by Bino, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de Castro, R. D.
Right arrow Articles by Bino, R. J.
Agricola
Right arrow Articles by de Castro, R. D.
Right arrow Articles by Bino, R. J.

PLANT PHYSIOLOGY , Vol 109, Issue 2 499-504, Copyright © 1995 by American Society of Plant Biologists

DEVELOPMENT AND GROWTH REGULATION

[beta]-Tubulin Accumulation and DNA Replication in Imbibing Tomato Seeds

R. D. de Castro, X. Zheng, JHW. Bergervoet, CHR. De Vos and R. J. Bino
Center for Plant Breeding and Reproduction Research, Department of Reproduction Technology, P.O. Box 16, 6700 AA Wageningen, The Netherlands (R.D.d.C., J.H.W.B., C.H.R.D.V., R.J.B.)

The activation of the cell cycle in embryo root tips of imbibing tomato (Lycopersicon esculentum Mill. cv Lerica) seeds was studied by flow cytometric analyses of the nuclear DNA content and by immunodelection of [beta]-tubulin. With dry seeds, flow cytometric profiles indicated that the majority of the cells were arrested at the G1 phase of the cell cycle. In addition, [beta]-tubulin was not detectable on western blots. Upon imbibition of water, the number of cells in G2 started to increase after 24 h, and a 55-kD [beta]-tubulin signal was detected between 24 and 48 h. Two-dimensional immunoblots revealed at least three different [beta]-tubulin isotypes. Thus, [beta]-tubulin accumulation and DNA replication were induced during osmotic priming. These processes, as well as seed germination rate, were enhanced upon subsequent imbibition of water, compared with control seeds that imbibed but were not primed. By contrast, when aged seeds imbibed, DNA replication, [beta]-tubulin accumulation, and germination were delayed. In all cases studied, both DNA replication and [beta]-tubulin accumulation preceded visible germination. We suggest that activation of these cell-cycle-related processes is a prerequisite for tomato seed germination. Furthermore, [beta]-tubulin expression can be used as a parameter for following the initial processes that are activated during seed imbibition.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
X. Q. Wang, P. F. Yang, Z. Liu, W. Z. Liu, Y. Hu, H. Chen, T. Y. Kuang, Z. M. Pei, S. H. Shen, and Y. K. He
Exploring the Mechanism of Physcomitrella patens Desiccation Tolerance through a Proteomic Strategy
Plant Physiology, April 1, 2009; 149(4): 1739 - 1750.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
E. A. A. Da Silva, P. E. Toorop, A. A. M. Van Lammeren, and H. W. M. Hilhorst
ABA Inhibits Embryo Cell Expansion and Early Cell Division Events During Coffee (Coffea arabica 'Rubi') Seed Germination
Ann. Bot., September 1, 2008; 102(3): 425 - 433.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
G. Bytof, S.-E. Knopp, D. Kramer, B. Breitenstein, J. H. W. Bergervoet, S. P. C. Groot, and D. Selmar
Transient Occurrence of Seed Germination Processes during Coffee Post-harvest Treatment
Ann. Bot., July 1, 2007; 100(1): 61 - 66.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. M. Barroco, K. Van Poucke, J. H.W. Bergervoet, L. De Veylder, S. P.C. Groot, D. Inze, and G. Engler
The Role of the Cell Cycle Machinery in Resumption of Postembryonic Development
Plant Physiology, January 1, 2005; 137(1): 127 - 140.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Soeda, M. C.J.M. Konings, O. Vorst, A. M.M.L. van Houwelingen, G. M. Stoopen, C. A. Maliepaard, J. Kodde, R. J. Bino, S. P.C. Groot, and A. H.M. van der Geest
Gene Expression Programs during Brassica oleracea Seed Maturation, Osmopriming, and Germination Are Indicators of Progression of the Germination Process and the Stress Tolerance Level
Plant Physiology, January 1, 2005; 137(1): 354 - 368.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
V Raz, J. Bergervoet, and M Koornneef
Sequential steps for developmental arrest in Arabidopsis seeds
Development, January 1, 2001; 128(2): 243 - 252.
[Abstract] [PDF]

Home page
 J Exp BotHome page
A. A. Powell, L. J. Yule, H.-C. Jing, S. P.C. Groot, R. J. Bino, and H. W. Pritchard
The influence of aerated hydration seed treatment on seed longevity as assessed by the viability equations
J. Exp. Bot., December 1, 2000; 51(353): 2031 - 2043.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. D. de Castro, A. A.M. van Lammeren, S. P.C. Groot, R. J. Bino, and H. W.M. Hilhorst
Cell Division and Subsequent Radicle Protrusion in Tomato Seeds Are Inhibited by Osmotic Stress But DNA Synthesis and Formation of Microtubular Cytoskeleton Are Not
Plant Physiology, February 1, 2000; 122(2): 327 - 336.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
L Russell, V Larner, S Kurup, S Bougourd, and M Holdsworth
The Arabidopsis COMATOSE locus regulates germination potential
Development, January 9, 2000; 127(17): 3759 - 3767.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1995 by the American Society of Plant Biologists