|
PLANT PHYSIOLOGY , Vol 109, Issue 2 619-625, Copyright © 1995 by American Society of Plant Biologists
|
BIOCHEMISTRY AND ENZYMOLOGY |
Molecular Cloning and Characterization of the cDNA Coding for the Biotin-Containing Subunit of the Chloroplastic Acetyl-Coenzyme A Carboxylase
J. K. Choi, F. Yu, E. S. Wurtele and B. J. Nikolau
Department of Biochemistry and Biophysics (B.J.N.), Department of Botany (F.Y., E.S.W.), and Department of Zoology and Genetics (J.-K.C.), Iowa State University, Ames, Iowa 50011
We report the molecular cloning and sequence of the cDNA coding for the
biotin-containing subunit of the chloroplastic acetylcoenzyme A (CoA)
carboxylase (ACCase) of Arabidopsis thaliana (CAC1). The 3[prime] end of
the CAC1 sequence, coding for a peptide of 94 amino acids, which includes a
putative biotinylation motif, was expressed in Escherichia coli as a
glutathione-S-transferase (GST) fusion protein. The resulting GST-CAC1
fusion protein was biotinylated in vivo, indicating that CAC1 codes for a
biotin-containing protein. Antibodies generated to the GST-CAC1 protein
reacted solely with the 38-kD biotin-containing polypeptide of Arabidopsis.
Furthermore, these antibodies inhibited ACCase activity in extracts from
Arabidopsis leaves. The deduced amino acid sequence of CAC1 has an apparent
N-terminal chloroplast-targeting transit peptide. The CAC1 protein is coded
by a single Arabidopsis gene, and its mRNA accumulates to the highest
levels in organs that are undergoing rapid growth. The amino acid sequence
of the CAC1 protein is most similar to the biotin carboxyl-carrier protein
component of eubacterial ACCases. These characterizations identify CAC1 as
the biotin-containing subunit of the plastidic, heteromeric ACCase of
Arabidopsis. The results support the ancient origin of the two structurally
distinct ACCases of plants.
This article has been cited by other articles:
|
|
|
|
 
B. L. Fatland, J. Ke, M. D. Anderson, W. I. Mentzen, L. W. Cui, C. C. Allred, J. L. Johnston, B. J. Nikolau, and E. S. Wurtele
Molecular Characterization of a Heteromeric ATP-Citrate Lyase That Generates Cytosolic Acetyl-Coenzyme A in Arabidopsis
Plant Physiology,
October 1, 2002;
130(2):
740 - 756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Denis, M. Grossemy, R. Douce, and C. Alban
Molecular Characterization of a Second Copy of Holocarboxylase Synthetase Gene (hcs2) in Arabidopsis thaliana
J. Biol. Chem.,
March 15, 2002;
277(12):
10435 - 10444.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Thelen, S. Mekhedov, and J. B. Ohlrogge
Brassicaceae Express Multiple Isoforms of Biotin Carboxyl Carrier Protein in a Tissue-Specific Manner
Plant Physiology,
April 1, 2001;
125(4):
2016 - 2028.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Ke, R. H. Behal, S. L. Back, B. J. Nikolau, E. S. Wurtele, and D. J. Oliver
The Role of Pyruvate Dehydrogenase and Acetyl-Coenzyme A Synthetase in Fatty Acid Synthesis in Developing Arabidopsis Seeds
Plant Physiology,
June 1, 2000;
123(2):
497 - 508.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. Ke, T.-N. Wen, B. J. Nikolau, and E. S. Wurtele
Coordinate Regulation of the Nuclear and Plastidic Genes Coding for the Subunits of the Heteromeric Acetyl-Coenzyme A Carboxylase
Plant Physiology,
April 1, 2000;
122(4):
1057 - 1072.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Mekhedov, O. M. de Ilárduya, and J. Ohlrogge
Toward a Functional Catalog of the Plant Genome. A Survey of Genes for Lipid Biosynthesis
Plant Physiology,
February 1, 2000;
122(2):
389 - 402.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Reverdatto, V. Beilinson, and N. C. Nielsen
A Multisubunit Acetyl Coenzyme A Carboxylase from Soybean
Plant Physiology,
March 1, 1999;
119(3):
961 - 978.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. Schulte, R. Topfer, R. Stracke, J. Schell, and N. Martini
Multi-functional acetyl-CoA carboxylase from Brassica napus is encoded by a multi-gene family: Indication for plastidic localization of at least one isoform
PNAS,
April 1, 1997;
94(7):
3465 - 3470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
