Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (39)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Waldo, G. S.
Right arrow Articles by Sayers, D. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Waldo, G. S.
Right arrow Articles by Sayers, D. E.
Agricola
Right arrow Articles by Waldo, G. S.
Right arrow Articles by Sayers, D. E.

PLANT PHYSIOLOGY , Vol 109, Issue 3 797-802, Copyright © 1995 by American Society of Plant Biologists

BIOCHEMISTRY AND ENZYMOLOGY

Formation of the Ferritin Iron Mineral Occurs in Plastids (An X-Ray Absorption Spectroscopy Study

G. S. Waldo, E. Wright, Z. H. Whang, J. F. Briat, E. C. Theil and D. E. Sayers
Department of Physics and Biochemistry, North Carolina State University, Raleigh, North Carolina 27695 (G.S.W., E.W., Z.-H.W., E.C.T., D.E.S)

Ferritin in plants is a nuclear-encoded, multisubunit protein found in plastids; an N-terminal transit peptide targets the protein to the plastid, but the site for formation of the ferritin Fe mineral is unknown. In biology, ferritin is required to concentrate Fe to levels needed by cells (approximately 10-7 M), far above the solubility of the free ion (10-18 M); the protein directs the reversible phase transition of the hydrated metal ion in solution to hydrated Fe-oxo mineral. Low phosphate characterizes the solid-phase Fe mineral in the center of ferritin of the cytosolic animal ferritin, but high phosphate is the hallmark of Fe mineral in prokaryotic ferritin and plant (pea [Pisum sativum L.] seed) ferritin. Earlier studies using x-ray absorption spectroscopy showed that high concentrations of phosphate present during ferritin mineralization in vitro altered the local structure of Fe in the ferritin mineral so that it mimicked the prokaryotic type, whether the protein was from animals or bacteria. The use of x-ray absorption spectroscopy to analyze the Fe environment in pea-seed ferritin now shows that the natural ferritin mineral in plants has an Fe-P interaction at 3.26A, similar to that of bacterial ferritin; phosphate also prevented formation of the longer Fe-Fe interactions at 3.5A found in animal ferritins or in pea-seed ferritin reconstituted without phosphate. Such results indicate that ferritin mineralization occurs in the plastid, where the phosphate content is higher; a corollary is the existence of a plastid Fe uptake system to allow the concentration of Fe in the ferritin mineral.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
L. Zheng, F. Huang, R. Narsai, J. Wu, E. Giraud, F. He, L. Cheng, F. Wang, P. Wu, J. Whelan, et al.
Physiological and Transcriptome Analysis of Iron and Phosphorus Interaction in Rice Seedlings
Plant Physiology, September 1, 2009; 151(1): 262 - 274.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Li, X. Fu, X. Qi, X. Hu, N. D. Chasteen, and G. Zhao
Protein Association and Dissociation Regulated by Ferric Ion: A NOVEL PATHWAY FOR OXIDATIVE DEPOSITION OF IRON IN PEA SEED FERRITIN
J. Biol. Chem., June 19, 2009; 284(25): 16743 - 16751.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
J.-F. Briat, K. Ravet, N. Arnaud, C. Duc, J. Boucherez, B. Touraine, F. Cellier, and F. Gaymard
New insights into ferritin synthesis and function highlight a link between iron homeostasis and oxidative stress in plants
Ann. Bot., May 29, 2009; (2009) mcp128v1.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
B. Lonnerdal
Soybean ferritin: implications for iron status of vegetarians
Am. J. Clinical Nutrition, May 1, 2009; 89(5): 1680S - 1685S.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. T. Ward, B. Lahner, E. Yakubova, D. E. Salt, and K. G. Raghothama
The Effect of Iron on the Primary Root Elongation of Arabidopsis during Phosphate Deficiency
Plant Physiology, July 1, 2008; 147(3): 1181 - 1191.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
B. Lonnerdal, A. Bryant, X. Liu, and E. C Theil
Iron absorption from soybean ferritin in nonanemic women
Am. J. Clinical Nutrition, January 1, 2006; 83(1): 103 - 107.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
E. C Theil and B. Lonnerdal
Reply to JR Hunt
Am. J. Clinical Nutrition, May 1, 2005; 81(5): 1179 - 1180.
[Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
P. Davila-Hicks, E. C Theil, and B. Lonnerdal
Iron in ferritin or in salts (ferrous sulfate) is equally bioavailable in nonanemic women
Am. J. Clinical Nutrition, October 1, 2004; 80(4): 936 - 940.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
L. E Murray-Kolb, R. Welch, E. C Theil, and J. L Beard
Women with low iron stores absorb iron from soybeans
Am. J. Clinical Nutrition, January 1, 2003; 77(1): 180 - 184.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J.-B. Peltier, G. Friso, D. E. Kalume, P. Roepstorff, F. Nilsson, I. Adamska, and K. J. van Wijk
Proteomics of the Chloroplast: Systematic Identification and Targeting Analysis of Lumenal and Peripheral Thylakoid Proteins
PLANT CELL, March 1, 2000; 12(3): 319 - 342.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1995 by the American Society of Plant Biologists