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PLANT PHYSIOLOGY , Vol 110, Issue 1 163-170, Copyright © 1996 by American Society of Plant Biologists

GENE REGULATION AND MOLECULAR GENETICS

Characterization of an Endo-[beta]-1,4-Glucanase Gene Induced by Auxin in Elongating Pea Epicotyls

S. C. Wu, J. M. Blumer, A. G. Darvill and P. Albersheim
Complex Carbohydrate Research Center and Department of Biochemistry and Molecular Biology, The University of Georgia, 220 Riverbend Road, Athens, Georgia 30602-4712

A gene (EGL1) encoding an endo-[beta]-1,4-D-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has been cloned and characterized. EGL1 encodes a 486-amino acid polypeptide, including a 24-mer putative signal peptide. The mature protein has a calculated molecular mass of 51.3 kD and an isoelectric point of 9.1. This pea EGase shares significant similarity with EGases from other plant species, but it appears to be distinct from the EGases associated with abscission and fruit ripening. Although EGL1 transcripts are detected in all parts of pea plants, they are relatively abundant in flowers and young pods undergoing rapid growth and most abundant in elongating epicotyls of etiolated seedlings. When epicotyl segments (6 mm long, 4 mm from the apical hook) are incubated in a 5 [mu]M solution of the synthetic auxin analog 2,4-dichlorophenoxyacetic acid, the concentration of EGL1 mRNA increases about 10-fold when the segments elongate most rapidly.


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