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PLANT PHYSIOLOGY , Vol 110, Issue 1 187-194, Copyright © 1996 by American Society of Plant Biologists
In Vitro Cyclic Electron Transport in Barley Thylakoids follows Two Independent Pathways
H. V. Scheller
Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, 40 Thorvaldsensvej, DK-1871 Frederiksberg C, Copenhagen, Denmark
In vitro cyclic electron transport around PSI was studied in thylakoids
isolated from barley (Hordeum vulgare L.). Redox poising was obtained by
using anaerobic conditions, preillumination, and the addition of
3-(3,4-dichlorophenyl)-1,1-dimethylurea. Postillumination rates of P700+
re-reduction of 1 to 5 electrons s-1 were observed, depending on the
conditions. The thylakoids supported two parallel paths of cyclic electron
transport that were distinguishable by differences in antimycin
sensitivity, saturation characteristics, and substrate specificity. The
pathway most sensitive to antimycin was not saturated at ferredoxin
concentrations up to 50 [mu]M, whereas the more insensitive pathway was
saturated at 5 [mu]M ferredoxin. At the lower concentration of reduced
ferredoxin, the antimycin-sensitive rate of P700+ re-reduction was lower
than the antimycin-insensitive rate. The lower range of reduced ferredoxin
concentrations are closer to in vivo conditions. Flavodoxin is shown to
mediate cyclic electron transport. Flavodoxin was less efficient in
mediating the antimycin-sensitive pathway but mediated the
antimycin-insensitive pathway as efficiently as ferredoxin. Antibodies
raised against ferredoxin:NADP+ oxidoreductase had no effect on either
pathway for re-reduction of P700+. However, the ferredoxin: NADP+
oxidoreductase inhibitor
2[prime]-monophosphoadenosine-5[prime]-diphosphoribose was able to inhibit
the antimycin-sensitive as well as the antimycin-insensitive pathway.
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