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PLANT PHYSIOLOGY , Vol 110, Issue 1 249-257, Copyright © 1996 by American Society of Plant Biologists
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WHOLE PLANT, ENVIRONMENTAL, AND STRESS PHYSIOLOGY |
Expression of a Late Embryogenesis Abundant Protein Gene, HVA1, from Barley Confers Tolerance to Water Deficit and Salt Stress in Transgenic Rice
D. Xu, X. Duan, B. Wang, B. Hong, THD. Ho and R. Wu
Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, New York 14853 (D.X., X.D., B.W., R.W.)
A late embryogenesis abundant (LEA) protein gene, HVA1, from barley
(Hordeum vulgare L.) was introduced into rice suspension cells using the
Biolistic-mediated transformation method, and a large number of independent
transgenic rice (Oryza sativa L.) plants were generated. Expression of the
barley HVA1 gene regulated by the rice actin 1 gene promoter led to
high-level, constitutive accumulation of the HVA1 protein in both leaves
and roots of transgenic rice plants. Second-generation transgenic rice
plants showed significantly increased tolerance to water deficit and
salinity. Transgenic rice plants maintained higher growth rates than
nontransformed control plants under stress conditions. The increased
tolerance was also reflected by delayed development of damage symptoms
caused by stress and by improved recovery upon the removal of stress
conditions. We also found that the extent of increased stress tolerance
correlated with the level of the HVA1 protein accumulated in the transgenic
rice plants. Using a transgenic approach, this study provides direct
evidence supporting the hypothesis that LEA proteins play an important role
in the protection of plants under water-or salt-stress conditions. Thus,
LEA genes hold considerable potential for use as molecular tools for
genetic crop improvement toward stress tolerance.
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