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PLANT PHYSIOLOGY , Vol 110, Issue 1 277-286, Copyright © 1996 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Genetic Modification of Respiratory Capacity in Potato
C. Hiser, P. Kapranov and L. McIntosh
Michigan State University-Department of Energy Plant Research Laboratory (C.H., P.K., L.M.) and Biochemistry Department, Michigan State University (L.M.), East Lansing, Michigan 28824
Mitochondrial respiration was altered in transgenic potato (Solanum
tuberosum) lines by overexpression of the alternative oxidase Aox1 gene.
Overexpressing lines showed higher levels of Aox1 mRNA, increased levels of
alternative oxidase protein(s), and an unusual higher molecular weight
polypeptide, which may be a normal processing/modification intermediate.
Evidence suggests that the alternative oxidase protein is further
processed/modified beyond removal of the transit peptide. Addition of
pyruvate to mitochondria oxidizing succinate or NADH increased the
alternative pathway capacity but did not eliminate the difference in the
capacity between these two substrates. Induction of alternative pathway
capacity by aging of tubers appeared to be more dependent on increased
levels of alternative oxidase protein than changes in its oxidation state.
In leaf and tuber mitochondria, overexpressing lines possessed higher
alternative pathway capacity than the control line, which suggests that
changing the alternative oxidase protein level by genetic engineering can
effectively change alternative pathway capacity.
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