|
PLANT PHYSIOLOGY , Vol 110, Issue 2 403-411, Copyright © 1996 by American Society of Plant Biologists
|
BIOCHEMISTRY AND ENZYMOLOGY |
Changing the Dimensions of Suberin Lamellae of Green Cotton Fibers with a Specific Inhibitor of the Endoplasmic Reticulum-Associated Fatty Acid Elongases
A. Schmutz, A. J. Buchala and U. Ryser
Institut fur Botanische Biologie, Universitat Freiburg, A. Gockelstrasse 3, CH-1700 Freiburg, Switzerland
The fibers of the green lint mutant of cotton (Gossypium hirsutum L.)
contain large amounts of wax and are suberized. More than 96% of the
bifunctional aliphatic suberin monomers ([alpha],[omega]-alkanedioic acids
and [omega]-hydroxyalkanoic acids) have chain lengths of C22 and C24 in
green cotton fiber suberin. In fibers grown in the presence of
S-ethyl-N,N-dipropylthiocarbamate (EPTC), a specific inhibitor of the
endoplasmic reticulum-associated fatty acid elongases, the aliphatic
suberin monomers were shortened to chain lengths of C16 and C18. Whereas
the amounts of most suberin monomers were not negatively affected by the
inhibitor treatment, the amounts of [alpha],[omega]-alkanedioic acids and
of glycerol were reduced by more than 80%. Analysis in the transmission
electron microscope showed a reduction in suberin content after EPTC
treatment. The suberin layers were discontinuous and consisted of fewer
lamellae than in the controls. A small proportion (up to 22%) of the
electron-translucent suberin lamellae were thinner after EPTC treatment,
probably because of the shortening of the aliphatic suberin monomers. A
larger proportion of the electron-translucent lamellae were thicker than
the lamellae in the controls. Possible explanations for this observation
are discussed.
This article has been cited by other articles:
|
|
|
|
 
I. Molina, Y. Li-Beisson, F. Beisson, J. B. Ohlrogge, and M. Pollard
Identification of an Arabidopsis Feruloyl-Coenzyme A Transferase Required for Suberin Synthesis
Plant Physiology,
November 1, 2009;
151(3):
1317 - 1328.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Serra, M. Soler, C. Hohn, R. Franke, L. Schreiber, S. Prat, M. Molinas, and M. Figueras
Silencing of StKCS6 in potato periderm leads to reduced chain lengths of suberin and wax compounds and increased peridermal transpiration
J. Exp. Bot.,
February 1, 2009;
60(2):
697 - 707.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Serra, M. Soler, C. Hohn, V. Sauveplane, F. Pinot, R. Franke, L. Schreiber, S. Prat, M. Molinas, and M. Figueras
CYP86A33-Targeted Gene Silencing in Potato Tuber Alters Suberin Composition, Distorts Suberin Lamellae, and Impairs the Periderm's Water Barrier Function
Plant Physiology,
February 1, 2009;
149(2):
1050 - 1060.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Panikashvili, S. Savaldi-Goldstein, T. Mandel, T. Yifhar, R. B. Franke, R. Hofer, L. Schreiber, J. Chory, and A. Aharoni
The Arabidopsis DESPERADO/AtWBC11 Transporter Is Required for Cutin and Wax Secretion
Plant Physiology,
December 1, 2007;
145(4):
1345 - 1360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li, F. Beisson, J. Ohlrogge, and M. Pollard
Monoacylglycerols Are Components of Root Waxes and Can Be Produced in the Aerial Cuticle by Ectopic Expression of a Suberin-Associated Acyltransferase
Plant Physiology,
July 1, 2007;
144(3):
1267 - 1277.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Moire, A. Schmutz, A. Buchala, B. Yan, R. E. Stark, and U. Ryser
Glycerol Is a Suberin Monomer. New Experimental Evidence for an Old Hypothesis
Plant Physiology,
March 1, 1999;
119(3):
1137 - 1146.
[Abstract]
[Full Text]
|
|
|
|
|
