PLANT PHYSIOLOGY , Vol 110, Issue 4 1145-1150, Copyright © 1996 by American Society of Plant Biologists

BIOCHEMISTRY AND ENZYMOLOGY

The Composition of Metals Bound to Class III Metallothionein (Phytochelatin and Its Desglycyl Peptide) Induced by Various Metals in Root Cultures of Rubia tinctorum

T. Maitani, H. Kubota, K. Sato and T. Yamada
National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya, Tokyo 158, Japan

The induction of phytochelatins (PCs) and their desglycyl peptides (both are referred to as class III metallothionein [CIIIMT]) by exposure to various metals (Ag+, As3+, As5+, Cd2+, Cu2+, Ga3+, Hg2+, In3+, Ni2+, Pb2+, Pd2+, Se4+, and Zn2+) and the metal composition in the CIIIMTs were investigated in root cultures of Rubia tinctorum L. All of these metal species induced PCs to various degrees when analyzed by the postcolumn derivatization high-performance liquid chromatography method. The desglycyl peptides of PCs often were also present. However, only Ag, Cd, and Cu were bound to the CIIIMTs that they induced when analyzed by the high-performance liquid chromatography-inductively coupled plasma-atomic emission spectrometry method. Cu was also bound to the CIIIMTs induced by Ag+, As3+, and Cd2+. After Ag+ exposure, an Fe peak that may be of Fe-CIIIMT was also observed. However, most of the metal species studied were not bound to the CIIIMTs that they induced.

This article has been cited by other articles:

				P. Tennstedt, D. Peisker, C. Bottcher, A. Trampczynska, and S. Clemens Phytochelatin Synthesis Is Essential for the Detoxification of Excess Zinc and Contributes Significantly to the Accumulation of Zinc Plant Physiology, February 1, 2009; 149(2): 938 - 948. [Abstract] [Full Text] [PDF]

				J. Ramos, L. Naya, M. Gay, J. Abian, and M. Becana Functional Characterization of an Unusual Phytochelatin Synthase, LjPCS3, of Lotus japonicus Plant Physiology, September 1, 2008; 148(1): 536 - 545. [Abstract] [Full Text] [PDF]

				S. Singh, A. Mulchandani, and W. Chen Highly Selective and Rapid Arsenic Removal by Metabolically Engineered Escherichia coli Cells Expressing Fucus vesiculosus Metallothionein Appl. Envir. Microbiol., May 1, 2008; 74(9): 2924 - 2927. [Abstract] [Full Text] [PDF]

				S. H. Kang, S. Singh, J.-Y. Kim, W. Lee, A. Mulchandani, and W. Chen Bacteria Metabolically Engineered for Enhanced Phytochelatin Production and Cadmium Accumulation Appl. Envir. Microbiol., October 1, 2007; 73(19): 6317 - 6320. [Abstract] [Full Text] [PDF]

				S. Iglesia-Turino, A. Febrero, O. Jauregui, C. Caldelas, J. L. Araus, and J. Bort Detection and Quantification of Unbound Phytochelatin 2 in Plant Extracts of Brassica napus Grown with Different Levels of Mercury Plant Physiology, October 1, 2006; 142(2): 742 - 749. [Abstract] [Full Text] [PDF]

				F. F. Nocito, C. Lancilli, B. Crema, P. Fourcroy, J.-C. Davidian, and G. A. Sacchi Heavy Metal Stress and Sulfate Uptake in Maize Roots Plant Physiology, July 1, 2006; 141(3): 1138 - 1148. [Abstract] [Full Text] [PDF]

				A. Chen, E. A. Komives, and J. I. Schroeder An Improved Grafting Technique for Mature Arabidopsis Plants Demonstrates Long-Distance Shoot-to-Root Transport of Phytochelatins in Arabidopsis Plant Physiology, May 1, 2006; 141(1): 108 - 120. [Abstract] [Full Text] [PDF]

				C. Ortega-Villasante, R. Rellan-Alvarez, F. F. Del Campo, R. O. Carpena-Ruiz, and L. E. Hernandez Cellular damage induced by cadmium and mercury in Medicago sativa J. Exp. Bot., August 1, 2005; 56(418): 2239 - 2251. [Abstract] [Full Text] [PDF]

				I. Kobayashi, S. Fujiwara, K. Shimogawara, C. Sakuma, Y. Shida, T. Kaise, H. Usuda, and M. Tsuzuki High Intracellular Phosphorus Contents Exhibit a Correlation with Arsenate Resistance in Chlamydomonas Mutants Plant Cell Physiol., March 1, 2005; 46(3): 489 - 496. [Abstract] [Full Text] [PDF]

				R. Ruotolo, A. Peracchi, A. Bolchi, G. Infusini, A. Amoresano, and S. Ottonello Domain Organization of Phytochelatin Synthase: FUNCTIONAL PROPERTIES OF TRUNCATED ENZYME SPECIES IDENTIFIED BY LIMITED PROTEOLYSIS J. Biol. Chem., April 9, 2004; 279(15): 14686 - 14693. [Abstract] [Full Text] [PDF]

				A. Raab, J. Feldmann, and A. A. Meharg The Nature of Arsenic-Phytochelatin Complexes in Holcus lanatus and Pteris cretica Plant Physiology, March 1, 2004; 134(3): 1113 - 1122. [Abstract] [Full Text] [PDF]

				A. L. Wangeline, J. L. Burkhead, K. L. Hale, S. D. Lindblom, N. Terry, M. Pilon, and E. A. H. Pilon-Smits Overexpression of ATP Sulfurylase in Indian Mustard: Effects on Tolerance and Accumulation of Twelve Metals J. Environ. Qual., January 1, 2004; 33(1): 54 - 60. [Abstract] [Full Text] [PDF]

				S. Sauge-Merle, S. Cuine, P. Carrier, C. Lecomte-Pradines, D.-T. Luu, and G. Peltier Enhanced Toxic Metal Accumulation in Engineered Bacterial Cells Expressing Arabidopsis thaliana Phytochelatin Synthase Appl. Envir. Microbiol., January 1, 2003; 69(1): 490 - 494. [Abstract] [Full Text] [PDF]

				H. Schat, M. Llugany, R. Vooijs, J. Hartley-Whitaker, and P. M. Bleeker The role of phytochelatins in constitutive and adaptive heavy metal tolerances in hyperaccumulator and non-hyperaccumulator metallophytes J. Exp. Bot., December 1, 2002; 53(379): 2381 - 2392. [Abstract] [Full Text] [PDF]

				J. Hartley-Whitaker, G. Ainsworth, R. Vooijs, W. T. Bookum, H. Schat, and A. A. Meharg Phytochelatins Are Involved in Differential Arsenate Tolerance in Holcus lanatus Plant Physiology, May 1, 2001; 126(1): 299 - 306. [Abstract] [Full Text]

				C. S. Cobbett Phytochelatins and Their Roles in Heavy Metal Detoxification Plant Physiology, July 1, 2000; 123(3): 825 - 832. [Full Text]

				M. E.V. Schmoger, M. Oven, and E. Grill Detoxification of Arsenic by Phytochelatins in Plants Plant Physiology, March 1, 2000; 122(3): 793 - 802. [Abstract] [Full Text] [PDF]

				S.-B. Ha, A. P. Smith, R. Howden, W. M. Dietrich, S. Bugg, M. J. O'Connell, P. B. Goldsbrough, and C. S. Cobbett Phytochelatin Synthase Genes from Arabidopsis and the Yeast Schizosaccharomyces pombe PLANT CELL, June 1, 1999; 11(6): 1153 - 1164. [Abstract] [Full Text]