PLANT PHYSIOLOGY , Vol 110, Issue 4 1431-1433, Copyright © 1996 by American Society of Plant Biologists
Appearance of Novel Glucose-6-Phosphate Dehydrogenase Isoforms in Chlamydomonas reinhardtii during Growth on Nitrate
H. C. Huppe and D. H. Turpin
Department of Biology, Queen's University, Kingston, Ontario, Canada K7L 3N6
Extractable glucose-6-phosphate dehydrogenase activity is higher from
N-limited Chlamydomonas reinhardtii cells than from N-sufficient cells.
Native gels reveal that the isoform complexity varies depending on the form
of N supplied. The isoforms associated with NO3- growth appear within 2 h
of switching cells from NH4+ to NO3-.