|
PLANT PHYSIOLOGY , Vol 111, Issue 1 227-233, Copyright © 1996 by American Society of Plant Biologists
|
WHOLE PLANT, ENVIRONMENTAL, AND STRESS PHYSIOLOGY |
Regulation of H+ Extrusion and Cytoplasmic pH in Maize Root Tips Acclimated to a Low-Oxygen Environment
J. H. Xia and JKM. Roberts
Department of Biochemistry, University of California, Riverside, California 92521
We tested the hypothesis that H+ extrusion contributes to cytoplasmic pH
regulation and tolerance of anoxia in maize (Zea mays) root tips. We
studied root tips of whole seedlings that were acclimated to a low-oxygen
environment by pretreatment in 3% (v/v) O2. Acclimated root tips
characteristically regulate cytoplasmic pH near neutrality and survive
prolonged anoxia, whereas nonacclimated tips undergo severe cytoplasmic
acidosis and die much more quickly. We show that the plasma membrane
H+-ATPase can operate under anoxia and that net H+ extrusion increases when
cytoplasmic pH falls. However, at an external pH near 6.0, H+ extrusion
contributes little to cytoplasmic pH regulation. At more acidic external pH
values, net H+ flux into root tips increases dramatically, leading to a
decrease in cytoplasmic pH and reduced tolerance of anoxia. We present
evidence that, under these conditions, H+ pumps are activated to partly
offset acidosis due to H+ influx and, thereby, contribute to cytoplasmic pH
regulation and tolerance of anoxia. The regulation of H+ extrusion under
anoxia is discussed with respect to the acclimation response and mechanisms
of intracellular pH regulation in aerobic plant cells.
This article has been cited by other articles:
|
|
|
|
 
K. Y. Kulichikhin, H. Greenway, L. Byrne, and T. D. Colmer
Regulation of intracellular pH during anoxia in rice coleoptiles in acidic and near neutral conditions
J. Exp. Bot.,
May 1, 2009;
60(7):
2119 - 2128.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. GREENWAY, W. ARMSTRONG, and T. D. COLMER
Conditions Leading to High CO2 (>5 kPa) in Waterlogged-Flooded Soils and Possible Effects on Root Growth and Metabolism
Ann. Bot.,
July 1, 2006;
98(1):
9 - 32.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. BAILEY-SERRES and R. CHANG
Sensing and Signalling in Response to Oxygen Deprivation in Plants and Other Organisms
Ann. Bot.,
September 1, 2005;
96(4):
507 - 518.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zhu, F. Yan, C. Zorb, and S. Schubert
A Link Between Citrate and Proton Release by Proteoid Roots of White Lupin (Lupinus albus L.) Grown Under Phosphorus-deficient Conditions?
Plant Cell Physiol.,
June 1, 2005;
46(6):
892 - 901.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Allegre, J. Silvestre, P. Morard, J. Kallerhoff, and E. Pinelli
Nitrate reductase regulation in tomato roots by exogenous nitrate: a possible role in tolerance to long-term root anoxia
J. Exp. Bot.,
December 1, 2004;
55(408):
2625 - 2634.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Gout, A.-M. Boisson, S. Aubert, R. Douce, and R. Bligny
Origin of the Cytoplasmic pH Changes during Anaerobic Stress in Higher Plant Cells. Carbon-13 and Phosphorous-31 Nuclear Magnetic Resonance Studies
Plant Physiology,
February 1, 2001;
125(2):
912 - 925.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W. W.P. Chang, L. Huang, M. Shen, C. Webster, A. L. Burlingame, and J. K.M. Roberts
Patterns of Protein Synthesis and Tolerance of Anoxia in Root Tips of Maize Seedlings Acclimated to a Low-Oxygen Environment, and Identification of Proteins by Mass Spectrometry
Plant Physiology,
February 1, 2000;
122(2):
295 - 318.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. W. Sowa, S. M. G. Duff, P. A. Guy, and R. D. Hill
Altering hemoglobin levels changes energy status in maize cells under hypoxia
PNAS,
August 18, 1998;
95(17):
10317 - 10321.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Yan, R. Feuerle, S. Schäffer, H. Fortmeier, and S. Schubert
Adaptation of Active Proton Pumping and Plasmalemma ATPase Activity of Corn Roots to Low Root Medium pH
Plant Physiology,
May 1, 1998;
117(1):
311 - 319.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Arpagaus, A. Rawyler, and R. Braendle
Occurrence and Characteristics of the Mitochondrial Permeability Transition in Plants
J. Biol. Chem.,
January 11, 2002;
277(3):
1780 - 1787.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
