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PLANT PHYSIOLOGY , Vol 111, Issue 1 339-345, Copyright © 1996 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Analysis of Flavanone 3-Hydroxylase in Arabidopsis Seedlings (Coordinate Regulation with Chalcone Synthase and Chalcone Isomerase)
M. K. Pelletier and B. W. Shirley
Department of Biology, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0406
A genomic clone encoding flavanone 3-hydroxylase (F3H) was isolated from
Arabidopsis thaliana. The deduced amino acid sequence is 72 to 94%
identical to all previously reported F3H proteins. Low-stringency DNA blot
analysis indicated that F3H is encoded by a single gene in Arabidopsis. The
F3H locus was mapped to the bottom of chromosome 3 and therefore does not
correspond to any of the 13 flavonoid-deficient transparent testa mutants
for which a map position is known. Analysis of gene expression in etiolated
seedlings exposed to white light and in two putative regulatory mutants,
ttg and tt8, demonstrated that the Arabidopsis F3H gene is coordinately
expressed with chalcone synthase and chalcone isomerase in seedlings,
whereas dihydroflavonol reductase expression is controlled by distinct
regulatory mechanisms. The F3H gene may represent a pivotal point in the
regulation of flavonoid biosynthesis because its expression is coordinated
with different subsets of genes in different plant species.
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