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PLANT PHYSIOLOGY , Vol 111, Issue 2 371-380, Copyright © 1996 by American Society of Plant Biologists
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CELL BIOLOGY AND SIGNAL TRANSDUCTION |
Modulation of Calmodulin mRNA and Protein Levels in Barley Aleurone
R. C. Schuurink, P. V. Chan and R. L. Jones
Department of Plant Biology, University of California, Berkeley, California 94720-3102
Changes in calmodulin (CaM) mRNA and protein were investigated in aleurone
layers of barley (Hordeum vulgare L. cv Himalaya) incubated in the presence
and absence of calcium, gibberellic acid (GA3), and abscisic acid (ABA).
CaM mRNA levels increased rapidly and transiently following incubation of
aleurone layers in H2O, CaCl2, or GA3. The increase in CaM mRNA was
prevented by ABA. This increase in CaM mRNA was brought about by physical
stimulation during removal of the starchy endosperm from the aleurone
layer. CaM protein levels did not increase in response to physical
stimulation. Only incubation in GA3 plus CaCl2 brought about a rapid
increase in CaM protein levels in the aleurone cell. ABA reduced the level
of CaM protein below that found at the beginning of the incubation period.
The rise in CaM protein preceded increases in the synthesis and secretion
of [alpha]-amylase. Immunocytochemistry with monoclonal antibodies to
carrot and mung bean CaM was used to localize CaM in aleurone protoplasts.
Monoclonal antibodies to tubulin and polyclonal antibodies to tonoplast
intrinsic protein and malate synthase were used as controls. CaM was
localized to the nucleus, the vacuolar membrane, and the cytosol, but was
not associated with microtubules.
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