|
PLANT PHYSIOLOGY , Vol 111, Issue 3 813-820, Copyright © 1996 by American Society of Plant Biologists
|
GENE REGULATION AND MOLECULAR GENETICS |
Pedicel Breakstrength and Cellulase Gene Expression during Tomato Flower Abscission
E. del Campillo and A. B. Bennett
Department of Plant Biology, University of Maryland, College Park, Maryland 20742 (E.d.C.)
Six cellulase genes were isolated from total RNA of the ethylene-treated
tomato (Lycopersicon esculentum Mill.) flower abscission zone by
reverse-transcription polymerase chain reaction using degenerate primers to
conserved amino acid sequences from known plant cellulases. Four of the
gene fragments are homologous to fruit pericarp cellulases. The other two
are novel cellulase genes, referred to as Cel5 and Cel6. Breakstrength and
cellulase gene expression were then analyzed in naturally abscising flowers
and flower explants. In both naturally abscising flowers and flower
explants induced to abscise in air or ethylene, both new cellulase mRNAs
were correlated with flower shedding. Whereas the Cel5 mRNA increased in
later stages of abscission, the Cel6 mRNA was present in nonabscising
flowers and then decreased in the final stage of abscission. A third
cellulase, Cel1, increased during the final stage of abscission in flower
explants and yet did not increase during shedding in planta, although it
was detectable at low levels in all abscission stages. Cel1 and Cel5 mRNA
decreased 99% when indole-3-acetic acid was added during ethylene
treatment, consistent with low levels of abscission (3%). In contrast, Cel6
mRNA increased slightly when indole-3-acetic acid was added. These results
suggest that abscission is a multistep process involving both activated and
repressed cellulase genes and that the relative importance of each
cellulase in the process depends on the physiological conditions under
which abscission takes place.
This article has been cited by other articles:
|
|
|
|
 
S. K. Tripathi, A. P. Singh, A. P. Sane, and P. Nath
Transcriptional activation of a 37 kDa ethylene responsive cysteine protease gene, RbCP1, is associated with protein degradation during petal abscission in rose
J. Exp. Bot.,
May 1, 2009;
60(7):
2035 - 2044.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Ogawa, P. Kay, S. Wilson, and S. M. Swain
ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 Are Polygalacturonases Required for Cell Separation during Reproductive Development in Arabidopsis
PLANT CELL,
January 1, 2009;
21(1):
216 - 233.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Agusti, P. Merelo, M. Cercos, F. R. Tadeo, and M. Talon
Ethylene-induced differential gene expression during abscission of citrus leaves
J. Exp. Bot.,
July 1, 2008;
59(10):
2717 - 2733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-Z. Jiang, F. Lu, W. Imsabai, S. Meir, and M. S. Reid
Silencing polygalacturonase expression inhibits tomato petiole abscission
J. Exp. Bot.,
March 2, 2008;
(2008)
ern023v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Trainotti, A. Pavanello, and D. Zanin
PpEG4 is a peach endo-{beta}-1,4-glucanase gene whose expression in climacteric peaches does not follow a climacteric pattern
J. Exp. Bot.,
February 1, 2006;
57(3):
589 - 598.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. D. Cin, M. Danesin, A. Boschetti, A. Dorigoni, and A. Ramina
Ethylene biosynthesis and perception in apple fruitlet abscission (Malus domestica L. Borck)
J. Exp. Bot.,
November 1, 2005;
56(421):
2995 - 3005.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. M. Fulton and C. S. Cobbett
Two {alpha}-L-arabinofuranosidase genes in Arabidopsis thaliana are differentially expressed during vegetative growth and flower development
J. Exp. Bot.,
November 1, 2003;
54(392):
2467 - 2477.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Molhoj, S. Pagant, and H. Hofte
Towards Understanding the Role of Membrane-bound Endo-{beta}-1,4-glucanases in Cellulose Biosynthesis
Plant Cell Physiol.,
December 15, 2002;
43(12):
1399 - 1406.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. H. Gonzalez-Carranza, C. A. Whitelaw, R. Swarup, and J. A. Roberts
Temporal and Spatial Expression of a Polygalacturonase during Leaf and Flower Abscission in Oilseed Rape and Arabidopsis
Plant Physiology,
February 1, 2002;
128(2):
534 - 543.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Goellner, X. Wang, and E. L. Davis
Endo-{beta}-1,4-Glucanase Expression in Compatible Plant-Nematode Interactions
PLANT CELL,
October 1, 2001;
13(10):
2241 - 2255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-B. Hong, R. Sexton, and M. L. Tucker
Analysis of Gene Promoters for Two Tomato Polygalacturonases Expressed in Abscission Zones and the Stigma
Plant Physiology,
July 1, 2000;
123(3):
869 - 882.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
I. Llop-Tous, E. Domínguez-Puigjaner, X. Palomer, and M. Vendrell
Characterization of Two Divergent Endo-beta -1,4-Glucanase cDNA Clones Highly Expressed in the Nonclimacteric Strawberry Fruit
Plant Physiology,
April 1, 1999;
119(4):
1415 - 1422.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. B. Smith
Of Abscission and Other Breakthroughs
PLANT CELL,
February 1, 1999;
11(2):
141 - 144.
[Full Text]
|
|
|
|
|
|
|
M. H. Harpster, D. A. Brummell, and P. Dunsmuir
Expression Analysis of a Ripening-Specific, Auxin-Repressed Endo-1,4-beta -Glucanase Gene in Strawberry
Plant Physiology,
December 1, 1998;
118(4):
1307 - 1316.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. A. Hadfield and A. B. Bennett
Polygalacturonases: Many Genes in Search of a Function
Plant Physiology,
June 1, 1998;
117(2):
337 - 343.
[Full Text]
|
|
|
|
|
|
|
D. A. Brummell, C. Catala, C. C. Lashbrook, and A. B. Bennett
A membrane-anchored E-type endo-1,4-beta -glucanase is localized on Golgi and plasma membranes of higher plants
PNAS,
April 29, 1997;
94(9):
4794 - 4799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
