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PLANT PHYSIOLOGY , Vol 111, Issue 3 885-891, Copyright © 1996 by American Society of Plant Biologists
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PLANT-MICROBE AND PLANT-INSECT INTERACTIONS |
Relationship between Active Oxygen Species, Lipid Peroxidation, Necrosis, and Phytoalexin Production Induced by Elicitins in Nicotiana
C. Rusterucci, V. Stallaert, M. L. Milat, A. Pugin, P. Ricci and J. P. Blein
Unite Associee Institut National de la Recherche Agronomique (INRA)-Universite de Bourgogne no. 692, Laboratoire de Phytopharmacie et Biochimie des Interactions Cellulaires, BV 1540, Dijon Cedex 21034, France (C.R., V.S., M.-L.M., A.P., J.-P.B.)
Excised leaves of Nicotiana tabacum var Xanthi and Nicotiana rustica were
treated with cryptogein and capsicein, basic and acidic elicitins,
respectively. Both compounds induced leaf necrosis, the intensity of which
depended on concentration and duration of treatment. N. tabacum var Xanthi
was the most sensitive species and cryptogein was the most active elicitin.
Lipid peroxidation in elicitin-treated Nicotiana leaves was closely
correlated with the appearance of necrosis. Elicitin treatments induced a
rapid and transient burst of active oxygen species (AOS) in cell cultures
of both Nicotiana species, with the production by Xanthi cells being 6-fold
greater than that by N. rustica. Similar maximum AOS production levels were
observed with both elicitins, but capsicein required 10-fold higher
concentrations than those of cryptogein. Phytoalexin production was lower
in response to both elicitins in N. tabacum var Xanthi cells than in N.
rustica cells, and capsicein was the most efficient elicitor of this
response. In cryptogein-treated cell suspensions, phytoalexin synthesis was
unaffected by diphenyleneiodonium, which inhibited AOS generation, nor was
it affected by tiron or catalase, which suppressed AOS accumulation in the
extracellular medium. These results suggest that AOS production, lipid
peroxidation, and necrosis are directly related, whereas phytoalexin
production depends on neither the presence nor the intensity of these
responses.
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