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PLANT PHYSIOLOGY , Vol 111, Issue 4 1011-1019, Copyright © 1996 by American Society of Plant Biologists
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DEVELOPMENT AND GROWTH REGULATION |
Isolation of Mutations Affecting the Development of Freezing Tolerance in Arabidopsis thaliana (L.) Heynh
G. Warren, R. McKown, A. Marin and R. Teutonico
DNA Plant Technology Corp., 6701 San Pablo Avenue, Oakland, California 94608
We screened for mutations deleterious to the freezing tolerance of
Arabidopsis thaliana (L.) Heynh. ecotype Columbia. Tolerance was assayed by
the vigor and regrowth of intact plants after cold acclimation and
freezing. From a chemically mutagenized population, we obtained 13 lines of
mutants with highly penetrant phenotypes. In 5 of these, freezing
sensitivity was attributable to chilling injury sustained during cold
acclimation, but in the remaining 8 lines, the absence of injury prior to
freezing suggested that they were affected specifically in the development
of freezing tolerance. In backcrosses, freezing sensitivity from each line
segregated as a single nuclear mutation. Complementation tests indicated
that the 8 lines contained mutations in 7 different genes. The mutants'
freezing sensitivity was also detectable in the leakage of electrolytes
from frozen leaves. However, 1 mutant line that displayed a strong
phenotype at the whole-plant level showed a relatively weak phenotype by
the electrolyte leakage assay.
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