Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (19)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Magnard, J. L.
Right arrow Articles by Dumas, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Magnard, J. L.
Right arrow Articles by Dumas, C.
Agricola
Right arrow Articles by Magnard, J. L.
Right arrow Articles by Dumas, C.

PLANT PHYSIOLOGY , Vol 111, Issue 4 1085-1096, Copyright © 1996 by American Society of Plant Biologists

DEVELOPMENT AND GROWTH REGULATION

Complexity and Genetic Variability of Heat-Shock Protein Expression in Isolated Maize Microspores

J. L. Magnard, P. Vergne and C. Dumas
Ecole Normale Superieure de Lyon, Reconnaissance Cellulaire et Amelioration des Plantes, Unite Mixte de Recherche Centre National de la Recherche Scientifique-Institut National de la Recherche Agronomique 9938, 69364 Lyon Cedex 07, France

The expression of heat-shock proteins (HSPs) in isolated maize (Zea mays L.) microspores has been investigated using high-resolution two-dimensional electrophoresis coupled to immunodetection and fluorography of in vivo synthesized proteins. To this end, homogeneous and viable populations of microspores have been purified in sufficient amounts for molecular analysis from plants grown in controlled conditions. Appropriate conditions for thermal stress application have been defined. The analysis revealed that isolated microspores from maize display a classical heat-shock response characterized by the repression of the normal protein synthesis and the expression of a set of HSPs. A high complexity of the response was demonstrated, with numerous different HSPs being resolved in each known major HSP molecular weight class. However, the extent of this heat-shock response is limited in that some of these HSPs do not accumulate at high levels following temperature elevation. Comparative analysis of the heat-shock responses of microspores isolated from five genotypes demonstrated high levels of genetic variability. Furthermore, many HSPs were detected in microspores at control temperature, indicating a possible involvement of these proteins in pollen development at stages close to first pollen mitosis.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
Y. Wang, W.-Z. Zhang, L.-F. Song, J.-J. Zou, Z. Su, and W.-H. Wu
Transcriptome Analyses Show Changes in Gene Expression to Accompany Pollen Germination and Tube Growth in Arabidopsis
Plant Physiology, November 1, 2008; 148(3): 1201 - 1211.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. Prasinos, K. Krampis, D. Samakovli, and P. Hatzopoulos
Tight regulation of expression of two Arabidopsis cytosolic Hsp90 genes during embryo development
J. Exp. Bot., February 1, 2005; 56(412): 633 - 644.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. V. Kuzmin, O. V. Karpova, T. E. Elthon, and K. J. Newton
Mitochondrial Respiratory Deficiencies Signal Up-regulation of Genes for Heat Shock Proteins
J. Biol. Chem., May 14, 2004; 279(20): 20672 - 20677.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Haralampidis, D. Milioni, S. Rigas, and P. Hatzopoulos
Combinatorial Interaction of Cis Elements Specifies the Expression of the Arabidopsis AtHsp90-1 Gene
Plant Physiology, July 1, 2002; 129(3): 1138 - 1149.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. E. Young, J. Ling, C. J. Geisler-Lee, R. L. Tanguay, C. Caldwell, and D. R. Gallie
Developmental and Thermal Regulation of the Maize Heat Shock Protein, HSP101
Plant Physiology, November 1, 2001; 127(3): 777 - 791.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
F. Schöffl, R. Prändl, and A. Reindl
Regulation of the Heat-Shock Response
Plant Physiology, August 1, 1998; 117(4): 1135 - 1141.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1996 by the American Society of Plant Biologists