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PLANT PHYSIOLOGY , Vol 112, Issue 1 281-290, Copyright © 1996 by American Society of Plant Biologists
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WHOLE PLANT, ENVIRONMENTAL, AND STRESS PHYSIOLOGY |
Nitrogen and Carbon Flows Estimated by 15N and 13C Pulse-Chase Labeling during Regrowth of Alfalfa
J. C. Avice, A. Ourry, G. Lemaire and J. Boucaud
Unite Associee Institut National de la Recherche Agronomique de Physiologie et de Biochimie Vegetales, Institut de Recherche en Biologie Appliquee, Universite de Caen, 14032 Caen Cedex, France (J.C.A., A.O., J.B.)
The flow of 15N and 13C from storage compounds in organs remaining after
defoliation (sources) to regrowing tissue (sinks), and 13C losses through
root or shoot respiration were assessed by pulse-chase labeling during
regrowth of alfalfa (Medicago sativa L.) following shoot removal. A total
of 73% of labeled C and 34% of labeled N were mobilized in source organs
within 30 d. Although all of the 15N from source organs was recovered in
the regrowing tissue, much of the 13C was lost, mainly as CO2 respired from
the root (61%) or shoot (8%), and was found to a lesser extent in sink
tissue (5%). After 3, 10, or 30 d of regrowth, 87, 66, and 52% of shoot N,
respectively, was derived from source tissue storage compounds; the rest
resulted from translocation of fixed N2. Overall results suggest that most
shoot C was linked to photosynthetic activity rather than being derived
from mobilization of stored C in source organs. Furthermore, isotopic
analysis of different chemical fractions of plant tissue suggests that
between 14 and 58% of the shoot C derived from source tissues was linked to
the mobilization of N compounds, not carbohydrates.
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