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PLANT PHYSIOLOGY , Vol 112, Issue 2 483-491, Copyright © 1996 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
The rab16B Promoter of Rice Contains Two Distinct Abscisic Acid-Responsive Elements
A. Ono, T. Izawa, N. H. Chua and K. Shimamoto
Plantech Research Institute, 1000 Kamoshida, Aoba-ku, Yokohama, 227 Japan (A.O., T.I., K.S.)
To localize abscisic acid (ABA)-inducible gene expression of rab16 genes,
rab16A promoter was linked to the gusA reporter gene encoding
[beta]-glucuronidase and introduced into rice (Oryza sativa L.) plants. The
activity of rab16A promoter was induced by ABA and osmotic stresses in
various tissues of vegetative and floral organs. In anthers and embryos,
rab16A promoter was active in the absence of ABA. To elucidate cis-elements
of the rab16 promoter that confer ABA-inducible expression, variously
modified 40-bp fragments (-264 to -225) of the rab16B promoter were fused
to a truncated (-46 bp) cauliflower mosaic virus 35S minimal promoter, and
their activities in protoplasts were analyzed. The transient assays
revealed that the 40-bp fragment consists of two separate ABA-responsive
elements, motif I (AGTACGTGGC) and motif III (GCCGCGTGGC). Motif I and
motif III are both required for ABA induction; however, each can substitute
for the other. Further analyses of these motifs indicated that motif III
has a distinct DNA sequence specificity as an ABA-responsive element from
motif I, suggesting that the two motifs interact with different
transcription factors in vivo.
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