PLANT PHYSIOLOGY , Vol 112, Issue 2 633-639, Copyright © 1996 by American Society of Plant Biologists
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BIOCHEMISTRY AND ENZYMOLOGY |
Cryopreservation of Chlorophyll Synthesis and Apoprotein Stabilization in Barley Etioplasts
L. A. Eichacker, I. Edhofer and G. Wanner
Department of Botany, University of Munich, 80638 Munich, Menzingerstrasse 67, Germany
Methods for the cryopreservation of protein import and integration in pea
chloroplasts and of protein import or protein synthesis in tobacco
mitochondria were modified to yield enzymatically active cryopreserved
etioplasts from barley (Hordeum vulgare L.). The cryoprotectants ethylene
glycol and dimethy sulfoxide were about 64 and 77% effective, respectively,
for the cryopreservation of etioplast intactness. Phototransformation of
protochlorophyllide a, esterification of chlorophyllide a or
zinc-pheophorbide a, and stabilization of the de novo synthesized
plastid-encoded chlorophyll-apoproteins P700, CP47, CP43, D2, and D1 were
successfully preserved in liquid nitrogen. Cryopreservation of freshly
prepared intact etioplasts completely retained enzymatic activities for
accumulation of chlorophyll a or resulted in a slightly decreased yield of
zinc-pheophytin a.
