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PLANT PHYSIOLOGY , Vol 112, Issue 2 735-745, Copyright © 1996 by American Society of Plant Biologists
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DEVELOPMENT AND GROWTH REGULATION |
Isolation and Characterization of Mutants of Arabidopsis thaliana with Increased Resistance to Growth Inhibition by Indoleacetic Acid-Amino Acid Conjugates
J. J. Campanella, J. Ludwig-Mueller and C. D. Town
Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106
Two mutants of Arabidopsis thaliana that are resistant to growth inhibition
by indole-3-acetic acid (IAA)-phenylalanine have been isolated. Both
mutants were 2- to 3-fold more resistant than wild type to inhibition by
IAA-phenylalanine, IAA-alanine, and IAA-glycine in root growth assays. The
mutant icr1 (but not icr2) also shows some resistance to IAA-aspartate.
Studies using 3H-labeled IAA-phenylalanine showed that the uptake of
conjugate from the medium by icr1 was the same as wild type and was reduced
by about 25% in icr2. No differences in hydrolysis of the exogenous
conjugate were detected between the mutants and their wild-type parents.
There was no significant metabolism of the IAA released from the
[3H]IAA-phenylalanine, whereas exogenous [3H]IAA was rapidly metabolized to
two unidentified products considerably more polar than IAA. Analysis of a
cross between icr1 and icr2 indicated that these mutations were at distinct
loci and that their effects were additive, and preliminary mapping data
indicated that icr1 and icr2 were located at the top and bottom of
chromosome V, respectively.
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