Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (84)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klann, E. M.
Right arrow Articles by Bennett, A. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klann, E. M.
Right arrow Articles by Bennett, A. B.
Agricola
Right arrow Articles by Klann, E. M.
Right arrow Articles by Bennett, A. B.

PLANT PHYSIOLOGY , Vol 112, Issue 3 1321-1330, Copyright © 1996 by American Society of Plant Biologists

WHOLE PLANT, ENVIRONMENTAL, AND STRESS PHYSIOLOGY

Antisense Acid Invertase (TIV1) Gene Alters Soluble Sugar Composition and Size in Transgenic Tomato Fruit

E. M. Klann, B. Hall and A. B. Bennett
Mann Laboratory, Department of Vegetable Crops, University of California, Davis, California 95616

Invertase ([beta]-fructosidase, EC 3.2.1.26) hydrolyzes sucrose to hexose sugars and thus plays a fundamental role in the energy requirements for plant growth and maintenance. Transgenic plants with altered extracellular acid invertase have highly disturbed growth habits. We investigated the role of intracellular soluble acid invertase in plant and fruit development. Transgenic tomato (Lycopersicon esculentum Mill.) plants expressing a constitutive antisense invertase transgene grew identically to wild-type plants. Several lines of transgenic fruit expressing a constitutive antisense invertase gene had increased sucrose and decreased hexose sugar concentrations. Each transgenic line with fruit that had increased sucrose concentrations also had greatly reduced levels of acid invertase in ripe fruit. Sucrose-accumulating fruit were approximately 30% smaller than control fruit, and this differential growth correlated with high rates of sugar accumulation during the last stage of development. These data suggest that soluble acid invertase controls sugar composition in tomato fruit and that this change in composition contributes to alterations in fruit size. In addition, sucrose-accumulating fruit have elevated rates of ethylene evolution relative to control fruit, perhaps as a result of the smaller fruit size of the sucrose-accumulating transgenic lines.


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
F. Mounet, A. Moing, V. Garcia, J. Petit, M. Maucourt, C. Deborde, S. Bernillon, G. Le Gall, I. Colquhoun, M. Defernez, et al.
Gene and Metabolite Regulatory Network Analysis of Early Developing Fruit Tissues Highlights New Candidate Genes for the Control of Tomato Fruit Composition and Development
Plant Physiology, March 1, 2009; 149(3): 1505 - 1528.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
X. Yu, X. Wang, W. Zhang, T. Qian, G. Tang, Y. Guo, and C. Zheng
Antisense suppression of an acid invertase gene (MAI1) in muskmelon alters plant growth and fruit development
J. Exp. Bot., August 1, 2008; 59(11): 2969 - 2977.
[Abstract] [Full Text] [PDF]

Home page
 jashsHome page
Y. Burger and A. A. Schaffer
The Contribution of Sucrose Metabolism Enzymes to Sucrose Accumulation in Cucumis melo
J. Amer. Soc. Hort. Sci., September 1, 2007; 132(5): 704 - 712.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Carrari and A. R. Fernie
Metabolic regulation underlying tomato fruit development
J. Exp. Bot., June 1, 2006; 57(9): 1883 - 1897.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. M. Eriksson, A. Bovy, K. Manning, L. Harrison, J. Andrews, J. De Silva, G. A. Tucker, and G. B. Seymour
Effect of the Colorless non-ripening Mutation on Cell Wall Biochemistry and Gene Expression during Tomato Fruit Development and Ripening
Plant Physiology, December 1, 2004; 136(4): 4184 - 4197.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
E. Urbanczyk-Wochniak, A. Leisse, U. Roessner-Tunali, A. Lytovchenko, J. Reismeier, L. Willmitzer, and A. R. Fernie
Expression of a Bacterial Xylose Isomerase in Potato Tubers Results in an Altered Hexose Composition and a Consequent Induction of Metabolism
Plant Cell Physiol., December 15, 2003; 44(12): 1359 - 1367.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Knapp
Tobacco to tomatoes: a phylogenetic perspective on fruit diversity in the Solanaceae
J. Exp. Bot., October 1, 2002; 53(377): 2001 - 2022.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Odanaka, A. B. Bennett, and Y. Kanayama
Distinct Physiological Roles of Fructokinase Isozymes Revealed by Gene-Specific Suppression of Frk1 and Frk2 Expression in Tomato
Plant Physiology, July 1, 2002; 129(3): 1119 - 1126.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Nguyen-Quoc and C. H. Foyer
A role for 'futile cycles' involving invertase and sucrose synthase in sucrose metabolism of tomato fruit
J. Exp. Bot., May 1, 2001; 52(358): 881 - 889.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. A. Hadfield, T. Dang, M. Guis, J.-C. Pech, M. Bouzayen, and A. B. Bennett
Characterization of Ripening-Regulated cDNAs and Their Expression in Ethylene-Suppressed Charentais Melon Fruit
Plant Physiology, March 1, 2000; 122(3): 977 - 984.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M.-A. D'Aoust, S. Yelle, and B. Nguyen-Quoc
Antisense Inhibition of Tomato Fruit Sucrose Synthase Decreases Fruit Setting and the Sucrose Unloading Capacity of Young Fruit
PLANT CELL, December 1, 1999; 11(12): 2407 - 2418.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
A. Sturm
Invertases. Primary Structures, Functions, and Roles in Plant Development and Sucrose Partitioning
Plant Physiology, September 1, 1999; 121(1): 1 - 8.
[Full Text]

Home page
 Plant CellHome page
G.-Q. Tang, M. Lüscher, and A. Sturm
Antisense Repression of Vacuolar and Cell Wall Invertase in Transgenic Carrot Alters Early Plant Development and Sucrose Partitioning
PLANT CELL, February 1, 1999; 11(2): 177 - 190.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1996 by the American Society of Plant Biologists