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PLANT PHYSIOLOGY , Vol 112, Issue 3 931-938, Copyright © 1996 by American Society of Plant Biologists

WHOLE PLANT, ENVIRONMENTAL, AND STRESS PHYSIOLOGY

Immunolocalization of Mannitol Dehydrogenase in Celery Plants and Cells

E. Zamski, Y. T. Yamamoto, J. D. Williamson, M. A. Conkling and D. M. Pharr
Departments of Horticultural Science (E.Z., D.M.P., J.D.W.) and Genetics (Y.T.Y, M.A.C.), North Carolina State University, Raleigh, North Carolina 27695

Immunolocalization of mannitol dehydrogenase (MTD) in celery (Apium graveolens L.) suspension cells and plants showed that MTD is a cytoplasmic enzyme. MTD was found in the meristems of celery root apices, in young expanding leaves, in the vascular cambium, and in the phloem, including sieve-element/companion cell complexes, parenchyma, and in the exuding phloem sap of cut petioles. Suspension cells that were grown in medium with mannitol as the sole carbon source showed a high anti-MTD cross-reaction in the cytoplasm, whereas cells that were grown in sucrose-containing medium showed little or no cross-reaction. Gel-blot analysis of proteins from vascular and nonvascular tissues of mature celery petioles showed a strong anti-MTD sera cross-reactive band, corresponding to the 40-kD molecular mass of MTD in vascular extracts, but no cross-reactive bands in nonvascular extracts. The distribution pattern of MTD within celery plants and in cell cultures that were grown on different carbon sources is consistent w ith the hypothesis that the Mtd gene may be regulated by sugar repression. Additionally, a developmental component may regulate the distribution of MTD within celery plants.


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Copyright © 1996 by the American Society of Plant Biologists