|
PLANT PHYSIOLOGY , Vol 112, Issue 4 1447-1456, Copyright © 1996 by American Society of Plant Biologists
|
GENE REGULATION AND MOLECULAR GENETICS |
Frequencies, Timing, and Spatial Patterns of Co-Suppression of Nitrate Reductase and Nitrite Reductase in Transgenic Tobacco Plants
J. C. Palauqui, T. Elmayan, F. D. de Borne, P. Crete, C. Charles and H. Vaucheret
Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, F-78026 Versailles Cedex, France
Frequencies, timing, and spatial patterns of co-suppression of the nitrate
(Nia) and nitrite (Nii) genes were analyzed in transgenic tobacco
(Nicotiana tabacum) plants carrying either Nia or Nii cDNAs under the
control of the 35S promoter, or a Nii gene with its own regulatory signals
(promoter, introns, and terminator) cloned downstream of two copies of the
enhancer of the 35S promoter. We show that (a) the frequencies of
transgenic lines affected by co- suppression are similar for the three
constructs, ranging from 19 to 25%; (b) Nia and Nii co-suppression are
triggered stochastically during a phenocritical period of 2 weeks between
germination and flowering; (c) the timing of co-suppression (i.e. the
percentage of isogenic plants affected by co-suppression reported as a
function of the number of days of culture) differs from one transgenic line
to another; (d) the percentage of isogenic plants affected by
co-suppression is increased by growing the plants in vitro prior to their
transfer to the greenhouse and to the field; and (e) at the end of the
culture period, plants are either unaffected, completely co-suppressed, or
variegated. Suppressed and nonsuppressed parts of these variegated plants
are separated by a vertical plane through the stem in Nia co-suppression,
and separated by a horizontal plane in Nii co-suppression.
This article has been cited by other articles:
|
|
|
|
 
E. De Paoli, A. Dorantes-Acosta, J. Zhai, M. Accerbi, D.-H. Jeong, S. Park, B. C. Meyers, R. A. Jorgensen, and P. J. Green
Distinct extremely abundant siRNAs associated with cosuppression in petunia
RNA,
November 1, 2009;
15(11):
1965 - 1970.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Wu and R. S. Poethig
Temporal regulation of shoot development in Arabidopsis thaliana by miR156 and its target SPL3
Development,
September 15, 2006;
133(18):
3539 - 3547.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Dubois, E. Botton, C. Meyer, A. Rieu, M. Bedu, B. Maisonneuve, and M. Mazier
Systematic silencing of a tobacco nitrate reductase transgene in lettuce (Lactuca sativa L.)
J. Exp. Bot.,
September 1, 2005;
56(419):
2379 - 2388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Petsch, J. Mylne, and J. R. Botella
Cosuppression of Eukaryotic Release Factor 1-1 in Arabidopsis Affects Cell Elongation and Radial Cell Division
Plant Physiology,
September 1, 2005;
139(1):
115 - 126.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Limpens, J. Ramos, C. Franken, V. Raz, B. Compaan, H. Franssen, T. Bisseling, and R. Geurts
RNA interference in Agrobacterium rhizogenes-transformed roots of Arabidopsis and Medicago truncatula
J. Exp. Bot.,
May 1, 2004;
55(399):
983 - 992.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. H. Tuteja, S. J. Clough, W.-C. Chan, and L. O. Vodkin
Tissue-Specific Gene Silencing Mediated by a Naturally Occurring Chalcone Synthase Gene Cluster in Glycine max
PLANT CELL,
April 1, 2004;
16(4):
819 - 835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Mlotshwa, O. Voinnet, M. F. Mette, M. Matzke, H. Vaucheret, S. W. Ding, G. Pruss, and V. B. Vance
RNA Silencing and the Mobile Silencing Signal
PLANT CELL,
May 1, 2002;
14(90001):
S289 - 301.
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Haywood, F. Kragler, and W. J. Lucas
Plasmodesmata: Pathways for Protein and Ribonucleoprotein Signaling
PLANT CELL,
May 1, 2002;
14(90001):
S303 - 325.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P.-Y. Teycheney and M. Tepfer
Virus-specific spatial differences in the interference with silencing of the chs-A gene in non-transgenic petunia
J. Gen. Virol.,
May 1, 2001;
82(5):
1239 - 1243.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. Vaucheret, C. Beclin, and M. Fagard
Post-transcriptional gene silencing in plants
J. Cell Sci.,
January 9, 2001;
114(17):
3083 - 3091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yamaguchi and M. Nishimura
Reduction to below Threshold Levels of Glycolate Oxidase Activities in Transgenic Tobacco Enhances Photoinhibition during Irradiation
Plant Cell Physiol.,
December 1, 2000;
41(12):
1397 - 1406.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Holtorf, H. Schob, C. Kunz, R. Waldvogel, and F. Meins , Jr.
Stochastic and Nonstochastic Post-Transcriptional Silencing of Chitinase and ß-1,3-Glucanase Genes Involves Increased RNA Turnover—Possible Role for Ribosome-Independent RNA Degradation
PLANT CELL,
March 1, 1999;
11(3):
471 - 484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R Ruiz-Medrano, B Xoconostle-Cazares, and W. Lucas
Phloem long-distance transport of CmNACP mRNA: implications for supracellular regulation in plants
Development,
January 10, 1999;
126(20):
4405 - 4419.
[Abstract]
[PDF]
|
|
|
|
|
|
|
J.-C. Palauqui and H. Vaucheret
Transgenes are dispensable for the RNA degradation step of cosuppression
PNAS,
August 4, 1998;
95(16):
9675 - 9680.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
