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PLANT PHYSIOLOGY , Vol 113, Issue 1 155-161, Copyright © 1997 by American Society of Plant Biologists
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DEVELOPMENT AND GROWTH REGULATION |
Endo-[beta]-Mannanase Activity Present in Cell Wall Extracts of Lettuce Endosperm prior to Radicle Emergence
S. Dutta, K. J. Bradford and D. J. Nevins
Department of Vegetable Crops, University of California, Davis, California 95616-8741
Lettuce (Lactuca sativa L.) endosperm cell walls isolated prior to radicle
emergence underwent autohydrolysis, the rate of which was correlated with
whether radicle emergence would subsequently occur. Extraction of endosperm
cell walls with 6 M LiCl suppressed autohydrolysis, and the desalted
extract possessed activity that was capable of hydrolyzing purified locust
bean galactomannan but not arabinogalactan, carboxymethylcellulose,
glucomannan, polygalacturonic acid, tomato galactomannan, or native lettuce
endosperm cell walls. Some hydrolytic activity was detected on endosperm
cell walls if they were modified by partial trifluoroacetic acid hydrolysis
or pretreatment with guanidinium thiocyanate. In extended incubations the
cell wall enzyme extract released only large molecular mass fragments from
locust bean galactomannan, indicating primarily endo-activity.
Galactomannan-hydrolyzing activity in the cell wall extract increased as a
function of imbibition time and was greatest just prior to radicle
emergence. Thermoinhibition (imbibition at 32[deg]C) or treatment with
abscisic acid at a temperature optimal for germination (25[deg]C)
suppressed both germination and endosperm cell wall mannanase activity,
whereas alleviation of thermoinhibition with gibberellic acid was
accompanied by significant enhancement of mannanase activity. We conclude
that a cell wall-bound endo-[beta]-mannanase is expressed in lettuce
endosperm prior to radicle emergence and is regulated by the same
conditions that govern germination.
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