|
PLANT PHYSIOLOGY , Vol 113, Issue 1 65-74, Copyright © 1997 by American Society of Plant Biologists
|
GENE REGULATION AND MOLECULAR GENETICS |
Molecular Cloning of 4-Coumarate:Coenzyme A Ligase in Loblolly Pine and the Roles of This Enzyme in the Biosynthesis of Lignin in Compression Wood
X. H. Zhang and V. L. Chiang
Plant Biotechnology Research Center, School of Forestry and Wood Products, Michigan Technological University, Houghton, Michigan 49931
Two genomic sequences encoding 4-coumarate:coenzyme A ligase (4CL;EC
6.2.1.12) in loblolly pine (Pinus taeda L.) were cloned. Both sequences
contained three introns and four exons with identical coding sequences
predicting 537 amino acids. Two of the three introns in these two clones
were different both in sequence and in length. Sequences of both 4CL clones
were found in all nine megagametophyte DNAs tested, providing genetic
evidence that these two 4CL genomic sequences are nonallelic genes. Our
analyses suggest that there are at least two distinct, intron-containing
4CL genes, at least one of which is transcribed into 4CL mRNA in developing
xylem tissue of loblolly pine. The levels of 4CL gene transcription in
xylem were influenced by compressional stress, resulting in an elevated 4CL
enzyme activity with 4-coumaric acid. 4CL enzyme activity with ferulic acid
remained unchanged, whereas with caffeic acid it was significantly
inhibited. Exogenously applied trans-cinnamic acid in the protein extracts
from normal wood xylem caused inhibition of 4CL activity toward caffeic
acid similar to that under compressional stress. The implications of this
cinnamic acid-modulated effect on 4CL enzyme activities toward different
substrates in regulating monolignol synthesis in xylem under compressional
stress are discussed.
This article has been cited by other articles:
|
|
|
|
 
A. Wagner, L. Donaldson, H. Kim, L. Phillips, H. Flint, D. Steward, K. Torr, G. Koch, U. Schmitt, and J. Ralph
Suppression of 4-Coumarate-CoA Ligase in the Coniferous Gymnosperm Pinus radiata
Plant Physiology,
January 1, 2009;
149(1):
370 - 383.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. C. Gonzalez-Martinez, N. C. Wheeler, E. Ersoz, C. D. Nelson, and D. B. Neale
Association Genetics in Pinus taeda L. I. Wood Property Traits
Genetics,
January 1, 2007;
175(1):
399 - 409.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Syring, A. Willyard, R. Cronn, and A. Liston
Evolutionary relationships among Pinus (Pinaceae) subsections inferred from multiple low-copy nuclear loci
Am. J. Botany,
December 1, 2005;
92(12):
2086 - 2100.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Jiang, K. V. Wood, and J. A. Morgan
Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae
Appl. Envir. Microbiol.,
June 1, 2005;
71(6):
2962 - 2969.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Peng, N. Misawa, and S. Harayama
Isolation and Characterization of Thermophilic Bacilli Degrading Cinnamic, 4-Coumaric, and Ferulic Acids
Appl. Envir. Microbiol.,
March 1, 2003;
69(3):
1417 - 1427.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lindermayr, J. Fliegmann, and J. Ebel
Deletion of a Single Amino Acid Residue from Different 4-Coumarate:CoA Ligases from Soybean Results in the Generation of New Substrate Specificities
J. Biol. Chem.,
January 24, 2003;
278(5):
2781 - 2786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Anterola, J.-H. Jeon, L. B. Davin, and N. G. Lewis
Transcriptional Control of Monolignol Biosynthesis in Pinus taeda. FACTORS AFFECTING MONOLIGNOL RATIOS AND CARBON ALLOCATION IN PHENYLPROPANOID METABOLISM
J. Biol. Chem.,
May 17, 2002;
277(21):
18272 - 18280.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Harding, J. Leshkevich, V. L. Chiang, and C.-J. Tsai
Differential Substrate Inhibition Couples Kinetically Distinct 4-Coumarate:Coenzyme A Ligases with Spatially Distinct Metabolic Roles in Quaking Aspen
Plant Physiology,
February 1, 2002;
128(2):
428 - 438.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Plomion, G. Leprovost, and A. Stokes
Wood Formation in Trees
Plant Physiology,
December 1, 2001;
127(4):
1513 - 1523.
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Li, X. F. Cheng, J. Leshkevich, T. Umezawa, S. A. Harding, and V. L. Chiang
The Last Step of Syringyl Monolignol Biosynthesis in Angiosperms Is Regulated by a Novel Gene Encoding Sinapyl Alcohol Dehydrogenase
PLANT CELL,
July 1, 2001;
13(7):
1567 - 1586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. J. McDougall
A comparison of proteins from the developing xylem of compression and non-compression wood of branches of Sitka spruce (Picea sitchensis) reveals a differentially expressed laccase
J. Exp. Bot.,
August 1, 2000;
51(349):
1395 - 1401.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Chen, H. Meyermans, B. Burggraeve, R. M. De Rycke, K. Inoue, V. De Vleesschauwer, M. Steenackers, M. C. Van Montagu, G. J. Engler, and W. A. Boerjan
Cell-Specific and Conditional Expression of Caffeoyl-Coenzyme A-3-O-Methyltransferase in Poplar
Plant Physiology,
July 1, 2000;
123(3):
853 - 868.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. Plomion, C. Pionneau, J. Brach, P. Costa, and H. Baillères
Compression Wood-Responsive Proteins in Developing Xylem of Maritime Pine (Pinus pinaster Ait.)
Plant Physiology,
July 1, 2000;
123(3):
959 - 970.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
X.-Q. Wang, D. C. Tank, and T. Sang
Phylogeny and Divergence Times in Pinaceae: Evidence from Three Genomes
Mol. Biol. Evol.,
May 1, 2000;
17(5):
773 - 781.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Li, J. L. Popko, T. Umezawa, and V. L. Chiang
5-Hydroxyconiferyl Aldehyde Modulates Enzymatic Methylation for Syringyl Monolignol Formation, a New View of Monolignol Biosynthesis in Angiosperms
J. Biol. Chem.,
February 25, 2000;
275(9):
6537 - 6545.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Osakabe, C. C. Tsao, L. Li, J. L. Popko, T. Umezawa, D. T. Carraway, R. H. Smeltzer, C. P. Joshi, and V. L. Chiang
Coniferyl aldehyde 5-hydroxylation and methylation direct syringyl lignin biosynthesis in angiosperms
PNAS,
August 3, 1999;
96(16):
8955 - 8960.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Allona, M. Quinn, E. Shoop, K. Swope, S. St. Cyr, J. Carlis, J. Riedl, E. Retzel, M. M. Campbell, R. Sederoff, et al.
Analysis of xylem formation in pine by cDNA sequencing
PNAS,
August 4, 1998;
95(16):
9693 - 9698.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-J. Hu, A. Kawaoka, C.-J. Tsai, J. Lung, K. Osakabe, H. Ebinuma, and V. L. Chiang
Compartmentalized expression of two structurally and functionally distinct 4-coumarate:CoA ligase genes in aspen (Populus tremuloides)
PNAS,
April 28, 1998;
95(9):
5407 - 5412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Allina, A. Pri-Hadash, D. A. Theilmann, B. E. Ellis, and C. J. Douglas
4-Coumarate:Coenzyme A Ligase in Hybrid Poplar . Properties of Native Enzymes, cDNA Cloning, and Analysis of Recombinant Enzymes
Plant Physiology,
February 1, 1998;
116(2):
743 - 754.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Li, J. L. Popko, X.-H. Zhang, K. Osakabe, C.-J. Tsai, C. P. Joshi, and V. L. Chiang
A novel multifunctional O-methyltransferase implicated in a dual methylation pathway associated with lignin biosynthesis in loblolly pine
PNAS,
May 13, 1997;
94(10):
5461 - 5466.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
