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PLANT PHYSIOLOGY , Vol 113, Issue 2 321-325, Copyright © 1997 by American Society of Plant Biologists
Cinnamyl Alcohol Dehydrogenase: Identification of New Sites of Promoter Activity in Transgenic Poplar
S. Hawkins, J. Samaj, V. Lauvergeat, A. Boudet and J. Grima-Pettenati
Centre de Physiologie et Biologie Vegetales, UMR Centre National de la Recherche Scientifique 5546, Universite Paul Sabatier, 118 route de Narbonne, 31062 Toulouse cedex, France
Stem sections from poplar that were stably transformed with a eucalypt
cinnamyl alcohol dehydrogenase promoter-[beta]-glucuronidase construct were
prepared by using either a technique routinely used in herbaceous species
or a technique designed to take into account the particular anatomy of
woody plants. Although both preparation techniques confirmed the pattern of
expression previously observed (C. Feuillet, V. Lauvergeat, C. Deswarte, G.
Pilate, A. Boudet and J. Grima-Pettenati [1995] Plant Mol Biol 27:
651-657), the latter technique also allowed the detection of other sites of
promoter activity not revealed by the first technique. In situ
hybridization confirmed the expression pattern obtained with the second
sample preparation technique.
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