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PLANT PHYSIOLOGY , Vol 113, Issue 2 357-365, Copyright © 1997 by American Society of Plant Biologists
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BIOCHEMISTRY AND ENZYMOLOGY |
Structure of the CAC1 Gene and in Situ Characterization of Its Expression (The Arabidopsis thaliana Gene Coding for the Biotin-Containing Subunit of the Plastidic Acetyl-Coenzyme A Carboxylase)
J. Ke, J. K. Choi, M. Smith, H. T. Horner, B. J. Nikolau and E. S. Wurtele
Department of Botany (J.K., M.S., H.T.H., E.S.W.), Department of Zoology and Genetics (J.-K.C.), and Department of Biochemistry and Biophysics (B.J.N.), Iowa State University, Ames, Iowa 50011
The CAC1 gene of Arabidopsis thaliana that codes for the biotin
carboxyl-carrier subunit of the heteromeric acetyl-coenzyme A carboxylase
was isolated and sequenced. CAC1 is a single-copy gene interrupted by six
introns. Subcellular immunogold labeling indicates that the biotin
carboxyl-carrier subunit is localized in the stroma of the plastids and
chloroplasts. The CAC1 mRNA accumulates throughout developing embryos and
ovules of siliques at a time of rapid growth and oil accumulation (7 d
after flowering), but is present at much lower levels in wall cells and
central septal cells of the silique. Immunolocalization studies show that
the pattern of accumulation of the biotin carboxyl-carrier subunit within
the siliques and leaves is similar to that of the CAC1 mRNA. These
observations indicate that the cellular pattern of biotin carboxyl-carrier
protein accumulation in the developing silique may be determined by the
transcriptional activity of the CAC1 gene.
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