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PLANT PHYSIOLOGY , Vol 113, Issue 3 987-995, Copyright © 1997 by American Society of Plant Biologists
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GENE REGULATION AND MOLECULAR GENETICS |
Identification of a 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene Linked to the Female (F) Locus That Enhances Female Sex Expression in Cucumber
T. Trebitsh, J. E. Staub and S. D. O'Neill
Section of Plant Biology, Division of Biological Sciences, University of California, Davis, California 95616 (T.T., S.D.O.)
Sex determination in cucumber (Cucumis sativus L.) is controlled largely by
three genes: F, m, and a. The F and m loci interact to produce monoecious
(M_f_) or gynoecious (M_F_) sex phenotypes. Ethylene and factors that
induce ethylene biosynthesis, such as 1-aminocyclopropane-1-carboxylate
(ACC) and auxin, also enhance female sex expression. A genomic sequence
(CS-ACS1) encoding ACC synthase was amplified from genomic DNA by a
polymerase chain reaction using degenerate oligonucleotide primers.
Expression of CS-ACS1 is induced by auxin, but not by ACC, in wounded and
intact shoot apices. Southern blot hybridization analysis of near-isogneic
gynoecious (MMFF) and monoecious (MMff) lines derived from diverse genetic
backgrounds revealed the existence of an additional ACC synthase (CS-ACS1G)
genomic sequence in the gynoecious lines. Sex phenotype analysis of a
segregating F2 population detected a 100% correlation between the CS-ACS1G
marker and the presence of the F locus. The CS-ACS1G gene is located in
linkage group B coincident with the F locus, and in the population tested
there was no recombination between the CS-ACS1G gene and the F locus.
Collectively, these data suggest that CS-ACS1G is closely linked to the F
locus and may play a pivotal role in the determination of sex in cucumber
flowers.
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