Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (98)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ma, J. F.
Right arrow Articles by Matsumoto, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ma, J. F.
Right arrow Articles by Matsumoto, H.
Agricola
Right arrow Articles by Ma, J. F.
Right arrow Articles by Matsumoto, H.

PLANT PHYSIOLOGY , Vol 113, Issue 4 1033-1039, Copyright © 1997 by American Society of Plant Biologists

WHOLE PLANT, ENVIRONMENTAL, AND STRESS PHYSIOLOGY

Internal Detoxification Mechanism of Al in Hydrangea (Identification of Al Form in the Leaves)

J. F. Ma, S. Hiradate, K. Nomoto, T. Iwashita and H. Matsumoto
Research Institute for Bioresources, Okayama University, Chuo 2-20-1, Kurashiki 710, Japan (J.F.M., H.M.)

An internal detoxification mechanism for Al was investigated in an Al-accumulating plant, hydrangea (Hydrangea macrophylla), focusing on Al forms present in the cells. The leaves of hydrangea contained as much as 15.7 mmol Al kg-1 fresh weight, and more than two-thirds of the Al was found in the cell sap. Using 27Al- nuclear magnetic resonance, the dominant peak of Al was observed at a chemical shift of 11 to 12 parts per million in both intact leaves and the extracted cell sap, which is in good accordance with the chemical shift for the 1:1 Al-citrate complex. Purification of cell sap by molecular sieve chromatography (Sephadex G-10) combined with ion-exclusion chromatography indicated that Al in fractions with the same retention time as citric acid contributed to the observed 27Al peak in the intact leaves. The molar ratio of Al to citric acid in the crude and purified cell sap approximated 1. The structure of the ligand chelated with Al was identified to be citric acid. Bioassay experiments showed that the purified Al complex from the cell sap did not inhibit root elongation of corn (Zea mays L.) and the viability of cells on the root tip surface was also not affected. These observations indicate that Al is bound to citric acid in the cells of hydrangea leaves.


This article has been cited by other articles:


Home page
 Plant CellHome page
C. F. Huang, N. Yamaji, N. Mitani, M. Yano, Y. Nagamura, and J. F. Ma
A Bacterial-Type ABC Transporter Is Involved in Aluminum Tolerance in Rice
PLANT CELL, February 1, 2009; 21(2): 655 - 667.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
K. M. Gabrielson, J. D. Cancel, L. F. Morua, and P. B. Larsen
Identification of dominant mutations that confer increased aluminium tolerance through mutagenesis of the Al-sensitive Arabidopsis mutant, als3-1
J. Exp. Bot., March 1, 2006; 57(4): 943 - 951.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Pineros, J. E. Shaff, H. S. Manslank, V. M. Carvalho Alves, and L. V. Kochian
Aluminum Resistance in Maize Cannot Be Solely Explained by Root Organic Acid Exudation. A Comparative Physiological Study
Plant Physiology, January 1, 2005; 137(1): 231 - 241.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. Shen, T. Iwashita, and J. F. Ma
Form of Al changes with Al concentration in leaves of buckwheat
J. Exp. Bot., January 1, 2004; 55(394): 131 - 136.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
S. JANSEN, T. WATANABE, and E. SMETS
Aluminium Accumulation in Leaves of 127 Species in Melastomataceae, with Comments on the Order Myrtales
Ann. Bot., July 1, 2002; 90(1): 53 - 64.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. A. Pineros, J. V. Magalhaes, V. M. Carvalho Alves, and L. V. Kochian
The Physiology and Biophysics of an Aluminum Tolerance Mechanism Based on Root Citrate Exudation in Maize
Plant Physiology, July 1, 2002; 129(3): 1194 - 1206.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
R. Shen and J. F. Ma
Distribution and mobility of aluminium in an Al-accumulating plant, Fagopyrum esculentum Moench.
J. Exp. Bot., August 1, 2001; 52(361): 1683 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
X. F. Li, J. F. Ma, and H. Matsumoto
Pattern of Aluminum-Induced Secretion of Organic Acids Differs between Rye and Wheat
Plant Physiology, August 1, 2000; 123(4): 1537 - 1544.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
I. R. Silva, T. J. Smyth, D. F. Moxley, T. E. Carter, N. S. Allen, and T. W. Rufty
Aluminum Accumulation at Nuclei of Cells in the Root Tip. Fluorescence Detection Using Lumogallion and Confocal Laser Scanning Microscopy
Plant Physiology, June 1, 2000; 123(2): 543 - 552.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
S. Jian Zheng, J. Feng Ma, and H. Matsumoto
High Aluminum Resistance in Buckwheat . I. Al-induced Specific Secretion of Oxalic Acid from Root Tips
Plant Physiology, July 1, 1998; 117(3): 745 - 751.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
J. Feng Ma, S. Hiradate, and H. Matsumoto
High Aluminum Resistance in Buckwheat . II. Oxalic Acid Detoxifies Aluminum Internally
Plant Physiology, July 1, 1998; 117(3): 753 - 759.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1997 by the American Society of Plant Biologists